A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans

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Mandelli, Fernanda ; Martins, Marcele Pandelo ; Chinaglia, Mariana ; de Lima, Evandro Antonio ; Morais, Mariana Abrahao Bueno ; Lima, Tatiani Brenelli ; Cabral, Lucelia ; Pirolla, Renan Augusto Siqueira ; Fuzita, Felipe Jun ; Paixao, Douglas Antonio Alvaredo ; Andrade, Maxuel de Oliveira ; Wolf, Lucia Daniela ; Vieira, Plinio Salmazo ; Persinoti, Gabriela Felix ; Murakami, Mario Tyago

• Tipo de documento: Artigo Científico
• Fonte: NPJ BIOFILMS AND MICROBIOMES; v. 10, n. 1, p. 13-pg., 2024-10-14.

Resumo

Gut microbiota members from the Bacteroidota phylum play a pivotal role in mammalian health and metabolism. They thrive in this diverse ecosystem due to their notable ability to cope with distinct recalcitrant dietary glycans via polysaccharide utilization loci (PULs). Our study reveals that a PUL from an herbivore gut bacterium belonging to the Bacteroidota phylum, with a gene composition similar to that in the human gut, exhibits extended functionality. While the human gut PUL targets mixed-linkage beta-glucans specifically, the herbivore gut PUL also efficiently processes linear and substituted beta-1,3-glucans. This gain of function emerges from molecular adaptations in recognition proteins and carbohydrate-active enzymes, including a beta-glucosidase specialized for beta(1,6)-glucosyl linkages, a typical substitution in beta(1,3)-glucans. These findings broaden the existing model for non-cellulosic beta-glucans utilization by gut bacteria, revealing an additional layer of functional and evolutionary complexity within the gut microbiota, beyond conventional gene insertions/deletions to intricate biochemical interactions. (AU)

• Processo FAPESP: 21/04891-3 - Mecanismos enzimáticos do microbioma de herbívoros aquáticos para a despolimerização e metabolismo de carboidratos complexos
• Beneficiário: Mário Tyago Murakami
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/03059-5 - Desvendando funções microbianas inéditas envolvidas na adaptação e processamento de poluentes sintéticos
• Beneficiário: Gabriela Felix Persinoti
• Modalidade de apoio: Auxílio à Pesquisa - Projeto Inicial
• Processo FAPESP: 21/09793-0 - Descoberta e investigação mecanística de enzimas ativas sobre carboidratos da microbiota intestinal do Peixe-Boi
• Beneficiário: Marcele Pandeló Martins
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado