Autor(es)

Fernandes, Andre J. ; Shibukawa, Vinicius P. ; Prata, Arnaldo M. R. ; Segato, Fernando ; dos Santos, Julio C. ; Ferraz, Andre ; Milagres, Adriane M. F.

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 401, p. 8-pg., 2024-04-29.

Resumo

Laccase isoforms from basidiomycetes exhibit a superior redox potential compared to commercially available laccases obtained from ascomycete fungi, rendering them more reactive toward mono -substituted phenols and polyphenolic compounds. However, basidiomycetes present limitations for large-scale culture in liquid media, restraining the current availability of laccases from this fungal class. To advance laccase production from basidiomycetes, a newly designed 14-L low -shear aerated and agitated bioreactor provided enzyme titers up to 23.5 IU/mL from Trametes versicolor cultures. Produced enzymes underwent ultrafiltration and LC/MS-MS characterization, revealing the predominant production of only two out of the ten laccases predicted in the T. versicolor genome. Process simulation and economic analysis using SuperPro designer (R) suggested that T. versicolor laccase could be produced at US$ 3.60/kIU in a 200-L/batch enterprise with attractive economic parameters and a payback period of 1.7 years. The study indicates that new bioreactors with plain design help to produce low-cost enzymes from basidiomycetes. (AU)

• Processo FAPESP: 19/25867-3 - Oxidação enzimática de lignosulfonatos e ácidos hidroxicinâmicos/xilana associada à preparação de adesivos
• Beneficiário: Andre Luis Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular