| Texto completo | |
| Autor(es): Mostrar menos - |
Pedrinha, Victor Feliz
;
Santos, Letrcia Martins
;
Goncalves, Carolina Passarelli
;
Garcia, Marra Terra
;
Lameira, Osmar Alves
;
Queiroga, Carmen Lucia
;
Marcucci, Maria Cristina
;
Shahbazi, Mohammad -Ali
;
Sharma, Prashant Kumar
;
Junqueira, Juliana Campos
;
Sipert, Carla Renata
;
de Andrade, Flaviana Bombarda
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMEDICINE & PHARMACOTHERAPY; v. 171, p. 14-pg., 2024-01-09. |
| Resumo | |
Root canal treatment addresses infectious processes that require control. Occasionally, the radicular pulp is vital and inflamed, presenting a superficial infection. To preserve pulpal remnants, conservative procedures have gained favor, employing anti-inflammatory medications. This study investigated the effects of propolis (PRO), and copaiba oil -resin (COR) associated with hydrocortisone (H) and compared their impact to that of Otosporin (R) concerning cytotoxic and genotoxic activity, cytokine detection, and toxicity in the Galleria mellonella model. Human periodontal ligament fibroblasts (PDLFs) were exposed to drug concentrations and evaluated by the MTT assay. Associations were tested from concentrations that did not compromise cell density. Genotoxicity was evaluated through micronucleus counting, while cytokines IL -6 and TGF-beta 1 were detected in the cell supernatant using ELISA. Molecular docking simulations were conducted, considering the major compounds identified in PRO, COR, and H. Increasing concentrations of PRO and COR were assessed for acute toxicity in Galleria mellonella model. Cellular assays were analyzed using one-way ANOVA followed by Tukey tests, while larval survivals were evaluated using the Log -rank (Mantel -Cox) test (alpha = 0.05). PRO and COR promoted PDLFs proliferation, even in conjunction with H. No changes in cell metabolism were observed concerning cytokine levels. The tested materials induce the release of AT1R, proliferating the PDFLs through interactions. PRO and COR had low toxicity in larvae, suggesting safety at tested levels. These findings endorse the potential of PRO and COR in endodontics and present promising applications across medical domains, such as preventive strategies in inflammation, shedding light on their potential development into commercially available drugs. (AU) | |
| Processo FAPESP: | 20/12726-0 - Papel da osteoprotegerina na modulação in vitro de monócitos por células de papila apical |
| Beneficiário: | Carla Renata Sipert |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/20186-3 - Fatores de adesão e biofilmes gerados por micro-organismos isolados de canais radiculares |
| Beneficiário: | Flaviana Bombarda de Andrade |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |