| Texto completo | |
| Autor(es): |
Cassas, Fernando
;
dos Reis, Vitor Eduardo Narciso
;
Cardoso, Carmen Lucia
;
Lopes, Thais de Assis
;
Cass, Quezia Bezerra
;
Uemi, Miriam
;
Vieira, Paulo Cezar
;
Barretto, Eduardo Hortal Pereira
;
de Medeiros, Livia Soman
;
Veiga, Thiago Andre Moura
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Fitoterapia; v. 175, p. 9-pg., 2024-04-24. |
| Resumo | |
Molecular dereplication and drug-like discovery are important tools for exploring the chemical profile of metabolites in a complex mixture. In order to establish a workflow for discovering novel acetylcholinesterase (AChE) ligands, we performed the chemical study of Myrsine guianensis (Aubl.) Kuntze (Primulaceae). To carry out the bioprospection, nine extracts were obtained from different parts of the plant. Through the dereplication approaches, seventeen metabolites were annotated. In order to confirm the putative inferences, a HPLC preparative method was developed to isolate three known myrsinoic acids, A (1), B (2) and C (3). Along with, we are reporting the obtention of two new congeners, G (5) and H (6), which their structures were elucidated by NMR and HRMS data. Besides that, two extracts were submitted to affinity assays to accelerate the discovery of AChE ligands. Desorbates were analyzed through LC-HRMS for calculating the affinity ratio (AR). Thus, (1) presented AR = 4.59, therefore was considered a potential ligand. (AU) | |
| Processo FAPESP: | 18/04095-0 - Abordagens analíticas para a descoberta de compostos citotóxicos desconhecidos a partir de plantas |
| Beneficiário: | Thiago André Moura Veiga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |