The synergistic effect of photodynamic and sonodynamic inactivation against Candida albicans biofilm

Autor(es)

Guimaraes, Gabriela Gomes ; Alves, Fernanda ; Goncalves, Isabella ; Carvalho, Iago Silva e ; Ayala, Erika Toneth Ponce ; Pratavieira, Sebastiao ; Bagnato, Vanderlei Salvador

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. 17, n. 9, p. 11-pg., 2024-07-17.

Resumo

Candida albicans biofilm can cause diseases that are resistant to conventional antifungal agents. Photodynamic (PDI), sonodynamic (SDI), and sonophotodynamic (SPDI) inactivation have arisen as promising antimicrobial strategies. This study evaluated these treatments mediated by curcumin against C. albicans biofilms. For this, C. albicans biofilms were submitted to PDI, SDI, or SPDI with different light and ultrasound doses, then, the viability assay was performed to measure the effectiveness. Finally, a mathematical model was suggested to fit acquired experimental data and understand the synergistic effect of light and ultrasound in different conditions. The results showed that SPDI, PDI, and SDI reduced the viability in 6 +/- 1; 1 +/- 1; and 2 +/- 1 log, respectively, using light at 60 J/cm(2), ultrasound at 3 W/cm(2), and 80 mu M of curcumin. The viability reduction was proportional to the ultrasound and light doses delivered. These results encourage the use of SPDI for the control of microbial biofilm. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 21/01324-0 - Desestruturação da matriz extracelular e uso de nanopartícula de curcumina para potencializar a inativação de biofilmes de Staphylococcus aureus
• Beneficiário: Fernanda Alves Dias de Sousa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático