Modulation of monocyte subtypes in diabetes after non-surgical periodontal treatment

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Alves, Jhefferson Miranda ; Germano, Danielle Borges ; Kim, Yeon Jung ; Fonseca, Francisco Antonio Helfenstein ; Izar, Maria Cristina ; Tuleta, Izabela Dorota ; Nagai, Rogerio ; Novo, Neil Ferreira ; Juliano, Yara ; Neves, Lucas Melo ; Pallos, Debora ; Franca, Carolina Nunes

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 27, n. 11, p. 8-pg., 2023-10-16.

Resumo

ObjectivesThe current study aims to evaluate the effect of non-surgical periodontal treatment on the modulation of monocyte phenotype, in the presence or absence of diabetes.Materials and methodsThe identification, quantification, and phenotypic characterization of monocyte subtypes (classical, intermediate, and non-classical) were performed by flow cytometry, at baseline and 1 month after the end of non-surgical periodontal treatment, in patients with periodontitis, associated or not with diabetes.ResultsThere was an increase in non-classical monocytes after treatment and a reduction in intermediate monocytes, without differences for the classical subtype, regardless of the diabetes status. Furthermore, there was a reduction in intermediate monocytes and an increase in non-classical and classical monocytes after treatment in the diabetes group, while no significant differences were observed for classical, intermediate, and non-classical monocytes in the group without diabetes. Comparisons between the two groups showed significant differences for classical, intermediate, and non-classical monocytes at baseline; these differences were not found one month after treatment.ConclusionsNon-surgical periodontal treatment leads to modulation of monocytes to a less inflammatory phenotype, especially in individuals with diabetes.Clinical relevance.A better understanding of the role of these biomarkers in the periodontitis contex may constitute a new strategic target for a better treatment of patiens with diabetes associated to periodontitis.Clinical Trial Registration.Brazilian Registry of Clinical Trials-RBR-35szwc.Jhefferson Miranda Alves and Danielle Borges Germano contributed equality to this study and should be considered first authors.ConclusionsNon-surgical periodontal treatment leads to modulation of monocytes to a less inflammatory phenotype, especially in individuals with diabetes.Clinical relevance.A better understanding of the role of these biomarkers in the periodontitis contex may constitute a new strategic target for a better treatment of patiens with diabetes associated to periodontitis.Clinical Trial Registration.Brazilian Registry of Clinical Trials-RBR-35szwc.Jhefferson Miranda Alves and Danielle Borges Germano contributed equality to this study and should be considered first authors.ConclusionsNon-surgical periodontal treatment leads to modulation of monocytes to a less inflammatory phenotype, especially in individuals with diabetes.Clinical relevance.A better understanding of the role of these biomarkers in the periodontitis contex may constitute a new strategic target for a better treatment of patiens with diabetes associated to periodontitis.Clinical Trial Registration.Brazilian Registry of Clinical Trials-RBR-35szwc.Jhefferson Miranda Alves and Danielle Borges Germano contributed equality to this study and should be considered first authors.ConclusionsNon-surgical periodontal treatment leads to modulation of monocytes to a less inflammatory phenotype, especially in individuals with diabetes.Clinical relevance.A better understanding of the role of these biomarkers in the periodontitis contex may constitute a new strategic target for a better treatment of patiens with diabetes associated to periodontitis.Clinical Trial Registration.Brazilian Registry of Clinical Trials-RBR-35szwc.Jhefferson Miranda Alves and Danielle Borges Germano contributed equality to this study and should be considered first authors. ConclusionsNon-surgical periodontal treatment leads to modulation of monocytes to a less inflammatory phenotype, especially in individuals with diabetes.Clinical relevance.A better understanding of the role of these biomarkers in the periodontitis contex may constitute a new strategic target for a better treatment of patiens with diabetes associated to periodontitis.Clinical Trial Registration.Brazilian Registry of Clinical Trials-RBR-35szwc.Jhefferson Miranda Alves and Danielle Borges Germano contributed equality to this study and should be considered first authors.ConclusionsNon-surgical periodontal treatment leads to modulation of monocytes to a less inflammatory phenotype, especially in individuals with diabetes.Clinical relevance.A better understanding of the role of these biomarkers in the periodontitis contex may constitute a new strategic target for a better treatment of patiens with diabetes associated to periodontitis.Clinical Trial Registration.Brazilian Registry of Clinical Trials-RBR-35szwc.Jhefferson Miranda Alves and Danielle Borges Germano contributed equality to this study and should be considered first authors. (AU)

• Processo FAPESP: 20/02254-3 - Papel das micropartículas circulantes na Doença Periodontal associada ao Diabetes
• Beneficiário: Karen Melissa Gonçalves Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/01020-1 - Papel dos subtipos de monócitos, micropartículas circulantes e quimiocinas na Doença Periodontal associada ao Diabetes
• Beneficiário: Carolina Nunes França
• Modalidade de apoio: Auxílio à Pesquisa - Regular