Autor(es)

de Melo, Gessica Baptista ; Roldan, William ; Malta, Fernanda de Mello ; Zevallos Lescano, Susana Angelica ; Castilho, Vera Lucia ; Goncalves, Elenice Messias Do Nascimento ; de Paula, Fabiana Martins ; Borges Gryschek, Ronaldo Cesar

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 62, p. 5-pg., 2020-01-01.

Resumo

Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova's medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3. 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp. (AU)

• Processo FAPESP: 15/18213-6 - Identificação de genótipos de Blastocystis spp. em amostras de fezes e sua relação com urticária crônica
• Beneficiário: Ronaldo Cesar Borges Cryschek
• Modalidade de apoio: Auxílio à Pesquisa - Regular