Antibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans

Autor(es)

Dias, Luana Mendonca ; Cilli, Eduardo Maffud ; Medeiros, Karine Sousa ; Brasil, Maria Carolina Oliveira de Arruda ; Marin, Lina Maria ; Siqueira, Walter L. ; Pavarina, Ana Claudia

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 12, n. 1, p. 21-pg., 2024-01-01.

Resumo

The aim of the study was to investigate the effect of antimicrobial peptides (AMPs) Hylin-a1, KR-12-a5, and Temporin-SHa in Candida albicans as well as the biocompatibility of keratinocytes spontaneously immortalized (NOK-si) and human gingival fibroblasts (FGH) cells. Initially, the susceptible (CaS-ATCC 90028) and fluconazole-resistant (CaR-ATCC 96901) C. albicans strains were grown to evaluate the effect of each AMP in planktonic culture, biofilm, and biocompatibility on oral cells. Among the AMPs evaluated, temporin-SHa showed the most promising results. After 24 h of Temporin-SHa exposure, the survival curve results showed that CaS and CaR suspensions reduced 72% and 70% of cell viability compared to the control group. The minimum inhibitory/fungicide concentrations (MIC and MFC) showed that Temporin-SHa was able to reduce >= 50% at >= 256 mu g/mL for both strains. The inhibition of biofilm formation, efficacy against biofilm formation, and total biomass assays were performed until 48 h of biofilm maturation, and Temporin-SHa was able to reduce >= 50% of CaS and CaR growth. Furthermore, Temporin-SHa (512 mu g/mL) was classified as non-cytotoxic and slightly cytotoxic for NOK-si and FGH, respectively. Temporin-SHa demonstrated an anti-biofilm effect against CaS and CaR and was biocompatible with NOK-si and FGH oral cells in monolayer. (AU)

• Processo FAPESP: 21/06476-3 - Análise do peptídeo Temporin-SHa sobre biofilme de Candida albicans e células da cavidade oral
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• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
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• Modalidade de apoio: Bolsas no Brasil - Doutorado