Genetic studies in Centrosema pubescens benth, a tropical forage legume: the mating system, genetic variability and genetic relationships between Centrosema species

Autor(es)

Sousa, A. C. B. ^[1] ; Carvalho, M. A. ^[2] ; Ramos, A. K. B. ^[2] ; Campos, T. ^[1] ; Sforca, D. A. ^[1] ; Zucchi, M. I. ^[3] ; Jank, L. ^[4] ; Souza, A. P. ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Genet Engn & Mol Biol Ctr CBMEG, BR-13083970 Campinas, SP - Brazil

^[2] Brazilian Agr Res Corp, Embrapa Cerrados, BR-73310970 Planaltina, DF - Brazil

^[3] Agron Inst Campinas, BR-13400970 Piracicaba, SP - Brazil

^[4] Brazilian Agr Res Corp, Forage Breeding Dept, EMBRAPA Beef Cattle, BR-79002970 Campo Grande, MS - Brazil

^[5] Univ Campinas UNICAMP, Plant Biol Dept DBV, Inst Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUPHYTICA; v. 181, n. 2, p. 223-235, SEP 2011.
• Citações Web of Science: 3

Resumo

In this study, we used microsatellite loci to estimate the outcrossing rate of Centrosema pubescens in open-pollinated populations of 10 progenies that each contained 20 genotypes. The multilocus outcrossing rate was 27%, which suggested a mixed mating system with a predominance of autogamy. The single locus outcrossing rate was 13%. The difference was 0.040, which indicated that only 4% of outcrossing occurs in related individuals. A paternity correlation of 14% suggests that there is a low probability of finding full sibs in the progeny. Cross-amplification of the 26 microsatellite loci available for C. pubescens was evaluated across 11 different Centrosema species. Nineteen of the 26 tested microsatellites were successfully transferable across the Centrosema species. The polymorphism information content and discriminating power evaluated had averages of 0.64 and 0.77, respectively. A total of three clusters were assembled to demonstrate the genetic relationships between Centrosema species. The transferable microsatellite loci should be useful for exploiting the genetic resources of the Centrosema species and determining the outcrossing rate, which are essential for proposing effective approaches for conservation and for establishing strategies for the selection and improvement of Centrosema spp. (AU)

• Processo FAPESP: 05/51010-0 - Desenvolvimento e caracterização de marcadores microssatélites em espécies forrageiras tropicais para utilização em estudos genéticos e melhoramento
• Beneficiário: Anete Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/52953-8 - Desenvolvimento e caracterização de marcadores moleculares microssatélites em forrageiras tropicais de interesse econômico
• Beneficiário: Adna Cristina Barbosa de Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado