| Texto completo | |
| Autor(es): |
Perin, Giovanni B.
;
Moreno, Silvia
;
Zhou, Yang
;
Guenther, Markus
;
Boye, Susanne
;
Voit, Brigitte
;
Felisberti, Maria I.
;
Appelhans, Dietmar
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biomacromolecules; v. 24, n. 12, p. 16-pg., 2023-11-20. |
| Resumo | |
In recent years, there has been growing attention to designing synthetic protocells, capable of mimicking micrometric and multicompartmental structures and highly complex physicochemical and biological processes with spatiotemporal control. Controlling metabolism-like cascade reactions in coacervate protocells is still challenging since signal transduction has to be involved in sequential and parallelized actions mediated by a pH change. Herein, we report the hierarchical construction of membraneless and multicompartmentalized protocells composed of (i) a cytosol-like scaffold based on complex coacervate droplets stable under flow conditions, (ii) enzyme-active artificial organelles and a substrate nanoreservoir capable of triggering a cascade reaction between them in response to a pH increase, and (iii) a signal transduction component based on the urease enzyme capable of the conversion of an exogenous biological fuel (urea) into an endogenous signal (ammonia and pH increase). Overall, this strategy allows a synergistic communication between their components within the membraneless and multicompartment protocells and, thus, metabolism-like enzymatic cascade reactions. This signal communication is transmitted through a scaffold protocell from an "inactive state" (nonfluorescent protocell) to an "active state" (fluorescent protocell capable of consuming stored metabolites). (AU) | |
| Processo FAPESP: | 15/25406-5 - Organizando a matéria: colóides formados por associação de surfactantes, polímeros e nanopartículas |
| Beneficiário: | Watson Loh |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 22/01139-1 - Construção de protocélulas biomiméticas baseadas em nanopartículas de poliéster e polimerossomos carregados de enzimas |
| Beneficiário: | Giovanni Bortoloni Perin |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto |
| Processo FAPESP: | 18/01562-6 - Polímeros inspirados em fosfolipídeos: poliésteres anfifílicos com grupo dihidrogenofosfato pendente |
| Beneficiário: | Giovanni Bortoloni Perin |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |