In vitro antimicrobial activity of non-traditional therapies for infectious endometritis in mares

Autor(es)

Mazzuchini, Mariana P. ; Lisboa, Fernando Paixao ; de Castro, Jessica I. ; Alvarenga, Marco A. ; Segabinazzi, Lorenzo G. T. M. ; Canisso, Igor F.

• Tipo de documento: Artigo Científico
• Fonte: EQUINE VETERINARY JOURNAL; v. N/A, p. 9-pg., 2024-10-21.

Resumo

Background: Endometritis is the leading cause of subfertility in horses, and it is a clinical problem where historically antibiotics have not always been used with prudent justification. Because of this, alternative therapies to treat endometritis are necessary for the rational use of antibiotics. Objectives: To assess the in vitro antimicrobial activity of non-traditional therapies commonly used in clinical practice against microorganisms causing infectious endometritis in mares. Study design: In vitro experiments. Methods: A microdilution technique was performed to determine the minimum inhibitory concentrations (MIC) of 50%, 90% and 100% of microorganisms and the percentage of inhibition (PI) of each therapy against each microorganism (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Candida sp.). The MIC 50% and 90% were determined using non-linear regression, while MIC 100% was assessed using the resazurin dye technique. The serial PI was evaluated for each therapy using a spectrophotometer. Results: All the therapies demonstrated a PI higher than positive controls for all microorganisms (p < 0.05); however, the only therapies that presented MIC 100 values were hydrogen peroxide and platelet-rich and -poor plasma. Main limitations: In vivo, safety and treatment efficacy were not tested. Conclusion: Hydrogen peroxide and platelet-rich and -poor plasma might be alternatives to traditional therapies for endometritis to support a reduction in antibiotic use. (AU)

• Processo FAPESP: 21/05152-0 - Efeito do plasma rico em plaquetas na resposta endometrial e microbiota do útero de éguas susceptíveis à endometrite e efeito antimicrobiano in vitro frente a bactérias causadoras de endometrite em éguas
• Beneficiário: Marco Antonio Alvarenga
• Modalidade de apoio: Auxílio à Pesquisa - Regular