| Texto completo | |
| Autor(es): |
Mazzuchini, Mariana P.
;
Lisboa, Fernando Paixao
;
de Castro, Jessica I.
;
Alvarenga, Marco A.
;
Segabinazzi, Lorenzo G. T. M.
;
Canisso, Igor F.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | EQUINE VETERINARY JOURNAL; v. N/A, p. 9-pg., 2024-10-21. |
| Resumo | |
Background: Endometritis is the leading cause of subfertility in horses, and it is a clinical problem where historically antibiotics have not always been used with prudent justification. Because of this, alternative therapies to treat endometritis are necessary for the rational use of antibiotics. Objectives: To assess the in vitro antimicrobial activity of non-traditional therapies commonly used in clinical practice against microorganisms causing infectious endometritis in mares. Study design: In vitro experiments. Methods: A microdilution technique was performed to determine the minimum inhibitory concentrations (MIC) of 50%, 90% and 100% of microorganisms and the percentage of inhibition (PI) of each therapy against each microorganism (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Candida sp.). The MIC 50% and 90% were determined using non-linear regression, while MIC 100% was assessed using the resazurin dye technique. The serial PI was evaluated for each therapy using a spectrophotometer. Results: All the therapies demonstrated a PI higher than positive controls for all microorganisms (p < 0.05); however, the only therapies that presented MIC 100 values were hydrogen peroxide and platelet-rich and -poor plasma. Main limitations: In vivo, safety and treatment efficacy were not tested. Conclusion: Hydrogen peroxide and platelet-rich and -poor plasma might be alternatives to traditional therapies for endometritis to support a reduction in antibiotic use. (AU) | |
| Processo FAPESP: | 21/05152-0 - Efeito do plasma rico em plaquetas na resposta endometrial e microbiota do útero de éguas susceptíveis à endometrite e efeito antimicrobiano in vitro frente a bactérias causadoras de endometrite em éguas |
| Beneficiário: | Marco Antonio Alvarenga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |