In situ interaction between the hormone 17α-ethynylestradiol and the liquid-ordered phase composed of the lipid rafts sphingomyelin and cholesterol

Autor(es)

Ruiz, Gilia Cristine Marques ; Morato, Luis Fernando do Carmo ; Pazin, Wallance Moreira ; Oliveira Jr, Osvaldo N. ; Constantino, Carlos Jose Leopoldo

• Tipo de documento: Artigo Científico
• Fonte: BIOORGANIC CHEMISTRY; v. 143, p. 8-pg., 2023-11-25.

Resumo

Hormone treatments are frequently associated with cardiovascular diseases and cancers in women. Additionally, the detrimental effects of their presence as contaminants in water remain a concern. The transport of hormones through cell membranes is essential for their biological action, but investigating cell permeability is challenging owing to the experimental difficulty in dealing with whole cells. In this paper, we study the interaction of the synthetic hormone 17 alpha-ethynylestradiol (EE2) with membrane models containing the key raft components sphingomyelin (SM) and cholesterol (Chol). The models consisted of Langmuir monolayers and giant unilamellar vesicles (GUVs) that represent bilayers. EE2 induced expansion of SM monolayers upon interacting with the nonhydrated amide group of SM head, but it had practically no effect on SM GUVs because these group are not available for interaction in bilayers. In contrast, EE2 interacted with hydrated phosphate group (PO2- ) and amide group of SM/Chol mixture monolayer, which could explain the loss in phase contrast of liquid-ordered GUVs suggesting pore formation. A comparison with reported EE2 effects on GUVs in the fluid phase, for which no loss in phase contrast was observed, indicates that the liquid-ordered phase consisting of lipid rafts is relevant to be associated with the changes on cell permeability caused by the hormones. (AU)

• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/15185-0 - Nanopartículas lipídicas para encapsulação e carreamento de produtos naturais: métodos de preparo, comparação e otimização
• Beneficiário: Gilia Cristine Marques Ruiz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado