| Texto completo | |
| Autor(es): |
Solis, Laura N. Fernandez
;
Silva Junior, Gilberto J.
;
Bertotti, Mauro
;
Angnes, Laicio
;
Pereira, Sirley V.
;
Fernandez-Baldo, Martin A. Fern
;
Regiart, Matias
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Talanta; v. 273, p. 8-pg., 2024-03-23. |
| Resumo | |
T-2 is one of the most potent cytotoxic food-borne mycotoxins. In this work, we have developed and characterized an electrochemical microfluidic immunosensor for T-2 toxin quantification in wheat germ samples. T-2 toxin detection was carried out using a competitive immunoassay method based on monoclonal anti-T-2 antibodies immobilized on the poly(methyl methacrylate) (PMMA) microfluidic central channel. The platinum wire working electrode at the end of the channel was in situ modified by a single-step electrodeposition procedure with reduced graphene oxide (rGO)-nanoporous gold (NPG). T-2 toxin in the sample was allowed to compete with T-2horseradish peroxidase (HRP) conjugated for the specific recognizing sites of immobilized anti-T-2 monoclonal antibodies. The HRP, in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of 4-tert-butylcatechol (4-TBC), whose back electrochemical reduction was detected on the nanostructured electrode at -0.15 V. Thus, at low T-2 concentrations in the sample, more enzymatically conjugated T-2 will bind to the capture antibodies, and, therefore, a higher current is expected. The detection limits found for electrochemical immunosensor, and commercial ELISA procedure were 0.10 mu g kg-1 and 10 mu g kg-1, and the intra- and inter-assay coefficients of variation were below 5.35% and 6.87%, respectively. Finally, our microfluidic immunosensor to T-2 toxin will significantly contribute to faster, direct, and secure in situ analysis in agricultural samples. (AU) | |
| Processo FAPESP: | 19/14418-3 - Sensores eletroquímicos para o monitoramento da qualidade de águas |
| Beneficiário: | Gilberto Jose Silva Junior |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |