Identification of Novel Immunoregulatory Molecules in Human Thymic Regulatory CD4(+)CD25(+) T Cells by Phage Display

Autor(es)

Porto, Georgia ^{[1, 2]} ; Giordano, Ricardo J. ^{[3, 4]} ; Marti, Luciana C. ^[5] ; Stolf, Beatriz ^{[6, 2]} ; Pasqualini, Renata ^[4] ; Arap, Wadih ^[4] ; Kalil, Jorge ^{[1, 2, 7]} ; Coelho, Veronica ^{[1, 2, 7]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Div Clin Immunol & Allergy, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Inst Coracao InCor, Inst Heart, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Chem, Sao Paulo - Brazil

^[4] Univ Texas MD Anderson Canc Ctr, David H Koch Ctr, Houston, TX 77030 - USA

^[5] Inst Israelita Ensino & Pesquisa Albert Einstein, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[7] Natl Inst Sci & Technol Iii INCT, Inst Invest Immunol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 6, n. 8 AUG 1 2011.
• Citações Web of Science: 7

Resumo

Thymic CD4(+)CD25(+) cells play an important role in immune regulation and are continuously developed in the thymus as an independent lineage. How these cells are generated, what are their multiple pathways of suppressive activity and which are their specific markers are questions that remain unanswered. To identify molecules involved in the function and development of human CD4(+)CD25(+) T regulatory cells we targeted thymic CD4(+)CD25(+) cells by peptide phage display. A phage library containing random peptides was screened ex vivo for binding to human thymic CD4(+)CD25(+) T cells. After four rounds of selection on CD4(+)CD25(+) enriched populations of thymocytes, we sequenced several phage displayed peptides and selected one with identity to the Vitamin D Receptor (VDR). We confirmed the binding of the VDR phage to active Vitamin D in vitro, as well as the higher expression of VDR in CD4(+)CD25(+) cells. We suggest that differential expression of VDR on natural Tregs may be related to the relevance of Vitamin D in function and ontogeny of these cells. (AU)

• Processo FAPESP: 03/04721-2 - Identificação de marcadores moleculares para células T reguladoras com perfil CD4+CD25+ por phage display
• Beneficiário: Georgia Sabio Porto Mundin
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto