| Texto completo | |
| Autor(es): |
Scharf, Pablo
;
Sandri, Silvana
;
Borges, Pamela Pacassa
;
de Oliveira, Tiago Franco
;
Farsky, Sandra Helena Poliselli
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Toxicology; v. 506, p. 5-pg., 2024-06-14. |
| Resumo | |
The toxicity of heated tobacco products (HTP) on the immune cells remains unclear. Here, U937 -differentiated macrophages were exposed to a single and short-term exposure (30 minutes) of HTP vapor or cigarette smoke (CS) in an air -liquid interface (ALI) system to evaluate the effects on macrophages' early activation and polarization. In our system, HTP released lower amounts of polycyclic aromatic hydrocarbons (PAHs), but higher nicotine levels than CS into the cell culture supernatant. Both tobacco products triggered the expression of the alpha-7 nicotinic receptor (alpha 7 nAChR) and reactive oxygen species (ROS) production. When challenged with a bacterial product, lipopolysaccharide (LPS), cells exposed to HTP or CS failed to respond properly and enhance ROS production upon LPS stimuli. Furthermore, both tobacco products also impaired bacterial phagocytosis and the exposures triggered higher IL-1 beta secretion. The alpha 7 nAChR antagonist treatment rescued the effects caused only by HTP exposure. The CS -exposed group switched macrophage to the pro -inflammatory M1, while HTP polarized to the suppressive M2 profile. Associated, data highlight that HTP and CS exposures similarly activate macrophages; nonetheless, the alpha 7 nAChR pathway is only involved in HTP actions, and the distinct subsequent polarization caused by HTP or CS may influence the outcome of host defense. (AU) | |
| Processo FAPESP: | 20/14368-3 - Efeitos da exposição a produtos de tabaco sobre linfócitos t cd4+ envolvidos na gênese da esclerose multipla |
| Beneficiário: | Pablo Rhansan dos Santos Scharf |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/19573-7 - Efeitos da exposição ao vapor aquecido do tabaco sobre a Artrite Reumatóide |
| Beneficiário: | Sandra Helena Poliselli Farsky |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/11779-8 - Mecanismos tóxicos dos produtos fumígenos sobre a produção e mobilização de neutrófilos em condições normais e na vigência de artrite reumatoide |
| Beneficiário: | Pâmela Pacassa Borges |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |