Micro/nanostructured carbon composite modified with a hybrid redox mediator and enzymes as a glucose biosensor

Autor(es)

Goncales, Vinicius Romero ^[1] ; Matsubara, Elaine Yoshiko ^[2] ; Rosolen, Jose Mauricio ^[2] ; Cordoba de Torresi, Susana Ines ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo - Brazil

^[2] Univ Sao Paulo, FFCLRP, Dept Quim, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Carbon; v. 49, n. 9, p. 3039-3047, AUG 2011.
• Citações Web of Science: 17

Resumo

A carbon micro/nanostructured composite based on cup-stacked carbon nanotubes (CSCNTs) grown onto a carbon felt has been found to be an efficient matrix for enzyme immobilization and chemical signal transduction. The obtained CSCNT/felt was modified with a copper hexacyanoferrate/polypyrrole (CuHCNFe/Ppy) hybrid mediator, and the resulting composite electrode was applied to H(2)O(2) detection, achieving a sensitivity of 194 +/- 15 mu A mmol(-1) L. The results showed that the CSCNT/felt matrix significantly increased the sensitivity of CuHCNFe/Ppy-based sensors compared to those prepared on a felt unrecovered by CSCNTs. Our data revealed that the improved sensitivity of the as-prepared CuHCNFe/Ppy-CSCNT/felt composite electrode can be attributed to the electronic interactions taking place among the CuHCNFe nanocrystals, Ppy layer and CSCNTs. In addition, the presence of CSCNTs also seemed to favor the dispersion of CuHCNFe nanocrystals over the Ppy matrix, even though the CSCNTs were buried under the conducting polymer layer. The CSCNT/felt matrix also enabled the preparation of a glucose biosensor whose sensitivity could be tuned as a function of the number of glucose oxidase (GOx) layers deposited through a Layer-by-Layer technique with an sensitivity of 11 +/- 2 mu A mmol(-1) L achieved at 15 poly(diallyldimethylammoniumchloride)/GOx bilayers. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 05/59560-9 - Nanoestruturação de filmes finos para utilização em eletrodos enzimáticos
• Beneficiário: Vinicius Romero Gonçales
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 06/07253-8 - Eletrodos compósitos de nanotubos de carbono de interesse para armazenagem de energia
• Beneficiário: Elaine Yoshiko Matsubara
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto