Autor(es)

Orzari, Luiz Otavio ; Guterres e Silva, Luiz Ricardo ; de Freitas, Rafaela Cristina ; Brazaca, Lais Canniatti ; Janegitz, Bruno Campos

• Tipo de documento: Artigo Científico
• Fonte: Microchimica Acta; v. 191, n. 1, p. 15-pg., 2024-01-01.

Resumo

A new conductive ink based on the addition of carbon black to a poly(vinyl alcohol) matrix is developed and investigated for electrochemical sensing and biosensing applications. The produced devices were characterized using morphological and electrochemical techniques and modified with Pd nanoparticles to enhance electrical conductivity and reaction kinetics. With the aid of chemometrics, the parameters for metal deposition were investigated and the sensor was applied to the determination of Parkinson's disease biomarkers, specifically epinephrine and alpha-synuclein. A linear behavior was obtained in the range 0.75 to 100 mu mol L-1 of the neurotransmitter, and the device displayed a limit of detection (LOD) of 0.051 mu mol L-1. The three-electrode system was then tested using samples of synthetic cerebrospinal fluid. Afterward, the device was modified with specific antibodies to quantify alpha-synuclein using electrochemical impedance spectroscopy. In phosphate buffer, a linear range was obtained for alpha-synuclein concentrations from 1.5 to 15 mu g mL(-1), with a calculated LOD of 0.13 mu g mL(-1). The proposed immunosensor was also applied to blood serum samples, and, in this case, the linear range was observed from 6.0 to 100.5 mu g mL(-1) of alpha-synuclein, with a LOD = 1.3 mu g mL(-1). Both linear curves attend the range for the real diagnosis, demonstrating its potential application to complex matrices. (AU)

• Processo FAPESP: 19/23342-0 - Desenvolvimento de novas arquiteturas de imunossensores para a detecção de biomarcadores das Doenças de Huntington e Parkinson
• Beneficiário: Luiz Otávio Orzari
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto