| Texto completo | |
| Autor(es): |
Fabri, Leonardo M.
;
Garcon, Daniela P.
;
Moraes, Cintya M.
;
Pinto, Marcelo R.
;
McNamara, John C.
;
Leone, Francisco A.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 268, p. 9-pg., 2023-08-02. |
| Resumo | |
This investigation examines the kinetic characteristics and effect of acclimation to a brackish medium (21 parts per thousand S) on gill V(H+)-ATPase activity in two hololimnetic populations of M. amazonicum. We also investigate the cellular immunolocalization of the enzyme. Immunofluorescence findings demonstrate that the V(H+)-ATPase c-subunit is distributed in the apical pillar cells of shrimps in fresh water but is absent after acclimation to 21 parts per thousand S for 10 days. V(H+)-ATPase activity from the Tiete River population is approximate to 50% greater than the Grande River population, comparable to a wild population from the Santa Elisa Reservoir, but is 2-fold less than in cultivated shrimps. V (H+)-ATPase activity in the Tiete and the Grande River shrimps is abolished after 21 parts per thousand S acclimation. The apparent affinities of the V(H+)-ATPase for ATP (0.27 +/- 0.04 and 0.16 +/- 0.03 mmol L-1, respectively) and Mg2+ (0.28 +/- 0.05 and 0.14 +/- 0.02 mmol L-1, respectively) are similar in both populations. The absence of V(H+)-ATPase activity in salinity-acclimated shrimps and its apical distribution in shrimps in fresh water underpins the importance of the crustacean V(H+)-ATPase for ion uptake in fresh water. (AU) | |
| Processo FAPESP: | 16/25336-0 - Estudo bioquímico comparativo da (Na+, K+)-ATPase branquial de Macrobrachium amazonicum (Heller, 1862) habitantes de regiões costeiras e continentais |
| Beneficiário: | Francisco de Assis Leone |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/21899-8 - Caracterização cinética e molecular da (Na+, K+)-ATPase branquial de Macrobrachium amazonicum (Heller, 1862) habitantes do Pantanal Matogrossense e da Bacia dos Rios Tocantins-Araguaia |
| Beneficiário: | Francisco de Assis Leone |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |