Voltammetric determination of uric acid using a miniaturized platform based on screen-printed electrodes modified with platinum nanoparticles

Autor(es)

Gomes-Junior, Paulo Cardoso ; Nascimento, Evair Dias ; Augusto, Karen Kenlderi de Lima ; Longatto, Gustavo Patelli ; Faria, Ronaldo Censi ; Piccin, Evandro ; Fatibello-Filho, Orlando

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 207, p. 8-pg., 2024-10-19.

Resumo

Disposable and sensitive screen-printed carbon electrodes (SPCE) modified with multi-walled carbon nanotubes (MWCNT) and platinum nanoparticles (PtNPs) were constructed to analyse uric acid (UA) in biological samples using a miniaturized smartphone potentiostat system. Combining the nanomaterials with NafionTM yielded a stable dispersion that could be successfully used as an electroactive layer. Transmission electron microscopy (TEM) and energy dispersive scanning (EDS) were used to characterize the morphology of PtNPs. These revealed approximately spherical shapes with emission lines characteristics of platinum. Furthermore, PtNPs/MWCNT/ SPCE film was characterized by scanning electron microscopy (SEM), which demonstrated the tubular characteristic of MWCNT distributed and covered by carbon black (CB) spheres present in the screen-printed ink. An enhanced current response was observed for UA voltammetric analysis, especially due to the synergism effect of PtNPs and MWCNT, which reduced the Rct value. The values of Dapp (1.6 x 10-6 cm2/s) and kcat (2.76 x 103 mol- 1 L/s) were determined for UA using the proposed sensor. The sensor displayed linear response for UA in the range from 5.0 x 10-6 mol/L to 6.9 x 10-4 mol/L, with a detection limit of 4.9 x 10- 7 mol/L. Along with detectability, excellent performances were verified in terms of repeatability, anti-interference features, and analysis of UA in the biological samples. (AU)

• Processo FAPESP: 22/15513-2 - Desenvolvimento de sensores eletroquímicos à base de material carbônico e de nanopartículas metálicas sintetizadas em solventes eutéticos profundos para a determinação de contaminantes emergentes
• Beneficiário: Gustavo Patelli Longatto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/22217-8 - Estudos termoanalíticos aplicados a fármacos antivirais antihepatite e antiretrovirais da classe dos inibidores de protease: comportamento térmico, intermediários de decomposição, caracterização de voláteis e polimorfismo
• Beneficiário: Éder Tadeu Gomes Cavalheiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 24/04116-8 - Emprego de solventes eutéticos profundos em procedimentos espectrofotométricos e eletroanalíticos sustentáveis
• Beneficiário: Orlando Fatibello Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular