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Marena, Gabriel Davi ; Lopez, Alejandro ; Carvalho, Gabriela Correa ; Marin, Maria del Pilar ; Ruiz, Maria Dolores Perez ; Perez-Royo, Jose Manuel ; Tormo-Mas, Maria angeles ; Bernabe, Patricia ; Valentin, Eulogio ; Bauab, Tais Maria ; Chorilli, Marlus ; Peman, Javier ; Ruiz-Gaitan, Alba

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS; v. 13, n. 7, p. 15-pg., 2024-07-01.

Resumo

Candida auris is an emerging, multidrug-resistant yeast that causes systemic infections, mainly in hospitalized or immunosuppressed patients. This pathogen has a high mortality and morbidity rate. This study aims to evaluate the antifungal potential of micafungin (MICA) encapsulated in a nanoemulsion (NEM) against four clades of C. auris and other non-C. auris species. The antifungal potential of MICA and NEM was evaluated by determining mature biofilm inhibition (0.78-50 mu g/mL). The antifungal activities of MICA and NEM (5.92 mg/Kg) were evaluated using an in vivo model of Galleria mellonella. The results showed that NEM intensified the antibiofilm action of MICA, especially in 48 h mature biofilms. In vivo results displayed a higher effectiveness of NEM against all clades of C. auris tested, inhibiting the fungal load in the hemolymph and tissues of G. mellonella with a difference of 3 log10. In addition, C. auris infection caused granulomas surrounded by hemocytes, mainly at the lower and upper ends. Conversely, C. albicans developed pseudohyphae, biofilms, filaments, and chlamydospores. In conclusion, encapsulation of MICA in a nanoemulsion enhances its antifungal activity against mature biofilms of C. auris. This strategy may be considered a therapeutic approach for the control of infections and the dissemination of this new global health threat. (AU)

• Processo FAPESP: 19/09831-9 - Nanoemulsões para co-encapsulação de anfotericina B e micafungina: desenvolvimento, caracterização e avaliação do potencial in vitro e in vivo contra Candida auris
• Beneficiário: Gabriel Davi Marena
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 21/11120-3 - Avaliação antifúngica da anfotericina B e micafungina co-encapsuladas em nanoemulsão contra isolados clínicos de Candida auris
• Beneficiário: Gabriel Davi Marena
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado