Autor(es)

de Godoy, Thais Almeida ; de Lima, Eduardo Oliveira Venancio ; Silveira, Giovanni Perez Machado ; Rodrigues, Fabiola Souza ; Sant'anna, Savio Stefani ; Hatakeyama, Daniela Miki ; Grego, Kathleen Fernandes ; Tanaka-Azevedo, Anita Mitico

• Tipo de documento: Artigo Científico
• Fonte: HELIYON; v. 10, n. 17, p. 12-pg., 2024-09-15.

Resumo

The genus Bothrops are considered Category 1 of medical importance by the World Health Organization, responsible for approximately 85 % of snakebites occurring throughout Brazil. Main factors determining snake venom variations can be genetics, diet, gender, geographic distribution, age, or even seasonality. In this study, we compared the composition of protein profile, biochemical activities, and immunorecognition of toxins present in the venom of eight adults of Bothrops species (B. alternatus, B. atrox, B. jararaca, B. jararacussu, B. leucurus, B. moojeni, B. neuwiedi and B. pauloensis). The following methods were used to analyze the venoms: protein dosage; electrophoresis in polyacrylamide gel containing SDS; High Performance Liquid Chromatography - Reverse Phase; enzymatic activities, western blotting and Enzyme Linked Immuno Sorbent Assay. The results show inter and intraspecific differences in the electrophoretic profile. LAAO and PLA(2) activities, in general, were higher in males than females and proteolytic activity was higher in females than males. The bothropic antivenom produced by Instituto Butantan recognized most of the protein bands in all Bothrops species analyzed, with only the regions between 37 and 25 kDa presenting lower intensity. A notable variability in the chromatograms was observed. Bothrops venom demonstrated inter-intraspecific disparities in protein composition and biochemical activity. (AU)

• Processo FAPESP: 22/15660-5 - Estudo ontogenético do veneno de serpentes Bothrops alternatus e análise de inibidores de fosfolipases A2 como terapia complementar à soroterapia em acidentes botrópicos
• Beneficiário: Eduardo Oliveira Venancio de Lima
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 21/11707-4 - Avaliação de métodos alternativos in vitro à utilização de animais para os ensaios in vivo no estudo de venenos de serpentes: princípio dos 3R's
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/14327-3 - 3r's e venômica: avaliação de um método in vitro alternativo ao uso de animais no estudo dos venenos de serpentes
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular