Parkinson biomarker determination with an Au microflower-enhanced electrochemical immunosensor using non-Faradaic capacitance measurements

Autor(es)

Orzari, Luiz Otavio ; Brazaca, Lais Canniatti ; Janegitz, Bruno Campos

• Tipo de documento: Artigo Científico
• Fonte: Microchimica Acta; v. 191, n. 11, p. 13-pg., 2024-11-01.

Resumo

This work comprehends the development and characterization of a carbon black-based electrode modified with Au microflowers to increase its effect as a capacitance biosensor for the determination of PARK7/DJ-1. Due to its high surface-to-volume ratio and biocompatibility, Au particles are suitable for antibody binding, and by monitoring surface capacitance, it is possible to identify the immune-pair interaction. Au microflowers allowed the adequate immobilization of Parkinsonian-related proteins: PARK7/DJ-1 and its antibody. The protein is associated with several antioxidant mechanisms, but its abnormal concentrations or mutations can be the cause of the loss of dopaminergic neurons, leading to Parkinson's disease. The device was characterized by scanning electron microscopy and cyclic voltammetry, revealing the flower-like structures and the electrochemically-interest enhancements they provide, such as increased heterogeneous electron transfer rate coefficient and electroactive area. The self-assembled monolayers of different molecules were optimized with the aid of 22 central composite experiments and a linear calibration curve was obtained between 0.700 and 120 ng mL-1 of PARK7/DJ-1, with a limit of detection of 0.207 ng mL-1. The data confirms that the addition of Au microflowers enhanced the electrochemical signal of the device, as well as allowed for the determination of an early stage Parkinson's disease biomarker with appreciable analytical performance. (AU)

• Processo FAPESP: 19/23342-0 - Desenvolvimento de novas arquiteturas de imunossensores para a detecção de biomarcadores das Doenças de Huntington e Parkinson
• Beneficiário: Luiz Otávio Orzari
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 23/10141-2 - Novos biossensores vestíveis para análises não invasivas de biomarcadores proteicos
• Beneficiário: Laís Canniatti Brazaca
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 23/06793-4 - Desenvolvimento de sistemas eletroquímicos multiplex aplicados para detecção de biomarcadores relacionados a doenças cardiovasculares
• Beneficiário: Bruno Campos Janegitz
• Modalidade de apoio: Auxílio à Pesquisa - Regular