Can the addition of Interleukin-13 affect the cryosurvival of bovine embryos?

Autor(es)

Valente, Roniele Santana ; Marsico, Thamiris Vieira ; Maiollo, Bruna Aparecida Prado ; Lopes, Nelyane Jose ; Tannura, Juliana Hayashi ; Sudano, Mateus Jose

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 215, p. 6-pg., 2023-12-08.

Resumo

In this study, we investigated the impact of incorporating Interleukin-13 (IL-13) into the embryonic culture medium and its influence on cryotolerance and cellular viability of vitrified bovine embryos. Two distinct time points for IL-13 supplementation were explored: during the final hours of culture prior to cryopreservation and during the period of recultivation following cryopreservation and warming. Cryosurvival rates, total cell count, and cell viability were assessed using the TUNEL technique to determine the apoptotic percentage. Re-expansion and hatching rates did not show differences among all groups (P > 0.05), and the total cell number was com-parable between the treated and control groups (P > 0.05). However, the group that received IL-13 before vitrification exhibited a higher apoptotic percentage (P < 0.05). This suggests that the anti-inflammatory effect of IL-13 may have impacted the embryo's defense capacity against the stress induced by cryopreservation, leading to an increased percentage of apoptosis, although it did not influence the developmental resumption capability. (AU)

• Processo FAPESP: 21/01446-9 - EMU concedido no processo 18/21496-8: Microscópio Invertido de Fluorescência e Contraste de interferência Diferencial (DIC)
• Beneficiário: Mateus José Sudano
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 19/17796-9 - Biopsia simultânea e perfil lipídico da massa celular interna e trofectoderma embrionário
• Beneficiário: Thamiris Vieira Marsico
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/21496-8 - Caminhos para prenhez embrionária
• Beneficiário: Mateus José Sudano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/18415-9 - Papel das vias inflamatórias sobre a competência e criotolerância embrionária
• Beneficiário: Roniele Santana Valente
• Modalidade de apoio: Bolsas no Brasil - Doutorado