| Texto completo | |
| Autor(es): |
Macedo, Tatiane Tiemi
;
Malavazi, Larissa Matias
;
Vargas, Gustavo Quilles
;
Goncalves, Francisco Jerfeson dos Santos
;
Gomes, Aline Paim de Abreu Paulo
;
Bueno, Manuela Rocha
;
Aguiar da Silva, Lucas Daylor
;
Figueiredo, Luciene Cristina
;
Bueno-Silva, Bruno
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMEDICINES; v. 12, n. 6, p. 9-pg., 2024-06-01. |
| Resumo | |
The aim of this study was to evaluate the effect of the combination of neovestitol-vestitol (CNV) compounds obtained from Brazilian red propolis on the microbiological profile of a mature multispecies subgingival biofilm. The biofilm with 32 bacterial species associated with periodontitis was formed for seven days using a Calgary device. Treatment with CNV (1600, 800, 400, and 200 mu g/mL), amoxicillin (54 mu g/mL), and vehicle control was performed for 24 h on the last day of biofilm formation. Biofilm metabolic activity and DNA-DNA hybridization (checkerboard) assays were performed. The groups treated with CNV 1600 and amoxicillin reduced 25 and 13 species, respectively, compared to the control vehicle treatment (p <= 0.05); both reduced P. gingivalis, while only CNV reduced T. forsythia. When the data from the two treatments (CNV and AMOXI) were compared, a statistically significant difference was observed in 13 species, particularly members of Socransky's orange complex. Our results showed that CNV at 1600 mu g/mL showed the best results regarding the metabolic activity of mature biofilms and obtained a reduction in species associated with the disease, such as T. forsythia, showing a better reduction than amoxicillin. Therefore, CNV seems to be a promising alternative to eradicate biofilms and reduce their pathogenicity. (AU) | |
| Processo FAPESP: | 17/16377-7 - Avaliação do efeito de bactérias probióticas sobre a resposta imune contra a.actinomycetemcomitans: estudo em células epiteliais gengivais e em modelo experimental de infecção in vivo. |
| Beneficiário: | Manuela Rocha Bueno |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/19691-0 - Atividade de compostos bioativos da própolis vermelha brasileira sobre a doença periodontal |
| Beneficiário: | Bruno Bueno Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |