Insights on PRAME and osteosarcoma by means of gene expression profiling

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Caminada Toledo, Silvia Regina ^{[1, 2]} ; Zago, Marco Antonio ^{[3, 4]} ; Oliveira, Indhira Dias ^{[1, 2]} ; Proto-Siqueira, Rodrigo ^{[3, 4]} ; Okamoto, Oswaldo K. ^[5] ; Severino, Patricia ^[6] ; Vencio, Ricardo Z. N. ^[7] ; Gamba, Francine Tesser ^{[1, 2]} ; Silva, Wilson A. ^{[3, 7, 4]} ; Moreira-Filho, Carlos A. ^[8] ; Dalla Torre, Cristiane A. ^{[1, 2]} ; Seixas Alves, Maria Tereza ^{[9, 2]} ; Garcia-Filho, Reynaldo J. ^{[10, 2]} ; Simpson, Andrew J. G. ^[11] ; Petrilli, Antonio Sergio ^[2]

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^[1] Univ Fed Sao Paulo, Dept Morphol & Genet, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, Pediat Oncol Inst GRAACC, Dept Pediat, Genet Lab, BR-04023062 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Clin Med, BR-14049 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Cellbased Therapy, BR-14049 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Dept Genet & Evolutionary Biol, BR-14049 Ribeirao Preto, SP - Brazil

^[6] Albert Einstein Res & Educ Inst, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Dept Genet, BR-14049 Ribeirao Preto, SP - Brazil

^[8] Univ Sao Paulo, Dept Pediat, BR-14049 Ribeirao Preto, SP - Brazil

^[9] Univ Fed Sao Paulo, Dept Pathol, BR-04023062 Sao Paulo - Brazil

^[10] Univ Fed Sao Paulo, Dept Orthoped Surg & Traumatol, BR-04023062 Sao Paulo - Brazil

^[11] Ludwig Inst, New York, NY - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORTHOPAEDIC SCIENCE; v. 16, n. 4, p. 458-466, JUL 2011.
• Citações Web of Science: 4

Resumo

Osteosarcoma (OS) is the most frequent bone tumor in children and adolescents. Tumor antigens are encoded by genes that are expressed in many types of solid tumors but are silent in normal tissues, with the exception of placenta and male germ-line cells. It has been proposed that antigen tumors are potential tumor markers. The premise of this study is that the identification of novel OS-associated transcripts will lead to a better understanding of the events involved in OS pathogenesis and biology. We analyzed the expression of a panel of seven tumor antigens in OS samples to identify possible tumor markers. After selecting the tumor antigen expressed in most samples of the panel, gene expression profiling was used to identify osteosarcoma-associated molecular alterations. A microarray was employed because of its ability to accurately produce comprehensive expression profiles. PRAME was identified as the tumor antigen expressed in most OS samples; it was detected in 68% of the cases. Microarray results showed differences in expression for genes functioning in cell signaling and adhesion as well as extracellular matrix-related genes, implying that such tumors could indeed differ in regard to distinct patterns of tumorigenesis. The hypothesis inferred in this study was gathered mostly from available data concerning other kinds of tumors. There is circumstantial evidence that PRAME expression might be related to distinct patterns of tumorigenesis. Further investigation is needed to validate the differential expression of genes belonging to tumorigenesis-related pathways in PRAME-positive and PRAME-negative tumors. (AU)

• Processo FAPESP: 07/53869-3 - Investigação de eventos genéticos envolvidos no processo de tumorigênese do osteossarcoma
• Beneficiário: Silvia Regina Caminada de Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/12150-8 - Expressao genica e polimorfismo em osteossarcoma.
• Beneficiário: Silvia Regina Caminada de Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular