Comparative effects of direct plasma treatment and plasma-activated media on B16F10 cancer cells using a multipoint surface dielectric barrier discharge system

Autor(es)

Lima, Luan G. ; Marcondes, Michaela Shiotani ; Neto, Nilton F. Azevedo ; Queiroz, Rafaela C. ; Tada, Dayane B. ; Junior, Clodomiro Alves ; Pessoa, Rodrigo S.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYSICS D-APPLIED PHYSICS; v. 58, n. 13, p. 18-pg., 2025-03-31.

Resumo

Cancer remains a leading cause of mortality worldwide, necessitating novel, effective, and selective therapies. Cold atmospheric plasma generates reactive species that can selectively kill tumor cells while sparing healthy tissue. We developed a multipoint surface dielectric barrier discharge (SDBD) plasma system capable of simultaneously treating all wells in a 24-well plate, enhancing experimental throughput and consistency. We investigated both direct plasma treatment and indirect effects via plasma-activated media (PAM) on the viability of B16F10 melanoma cells and L929 fibroblasts. Characterizations confirmed uniform plasma generation and safe operating temperatures suitable for cell applications. Physicochemical analyses of PAMs showed time-dependent changes in pH, oxidation-reduction potential, and conductivity, indicating the generation of reactive species like nitrite, nitrate, and hydrogen peroxide. Cytotoxicity assays demonstrated that both direct plasma treatment and PAM significantly reduced melanoma cell viability, with direct treatment causing a more pronounced effect. After 3 min of direct exposure, melanoma cell viability decreased to approximately 30% after 24 h and further to 13% after 48 h. In contrast, healthy L929 fibroblasts remained largely unaffected under both treatment conditions. These results suggest that both short-lived and long-lived reactive species contribute to the selective cytotoxic effects observed in cancer cells. The multipoint SDBD plasma system shows potential as a selective anticancer treatment modality, effective through both direct plasma exposure and the application of PAM, offering a promising avenue for future cancer therapies. (AU)

• Processo FAPESP: 19/05856-7 - Aplicações de plasmas atmosféricos não-térmicos na odontologia: da bancada para a clínica
• Beneficiário: Cristiane Yumi Koga Ito
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/02410-3 - Aplicações emergentes de plasmas frios atmosféricos nas áreas médica e odontologica
• Beneficiário: Rodrigo Savio Pessoa
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Brasil