Swine clones: potential application for animal production and animal models

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Nesiyama, Thais Naomi Gonsalves ; Sangalli, Juliano Rodrigues ; De Bem, Tiago Henrique Camara ; Recchia, Kaiana ; Martins, Simone Maria Massami Kitamura ; de Andrade, Andre Furugen Cesar ; Ferst, Juliana Germano ; Almeida, Gustavo Henrique Dona Rodrigues ; Marques, Mariana Groke ; Doria, Renata Gebara Sampaio ; Carregaro, Adriano Bonfim ; Feliciano, Marcus Antonio Rossi ; Miglino, Maria Angelica ; Fernandes, Fabiana ; Perecin, Felipe ; da Silveira, Juliano Coelho ; Smith, Lawrence Charles ; Bordignon, Vilceu ; Meirelles, Flavio Vieira

• Tipo de documento: Artigo Científico
• Fonte: ANIMAL REPRODUCTION; v. 22, n. 1, p. 13-pg., 2025-01-01.

Resumo

Somatic cell nuclear transfer (SCNT), or cloning, is used to reprogram cells and generate genetically identical embryos and animals. However, the cloning process is inefficient, limiting its application to producing valuable animals. In swine, cloning is mainly utilized to produce genetically modified animals. Indeed, recombinant DNA technologies have evolved considerably in recent years, with homologous recombination and gene editing technologies becoming more efficient and capable of recombining both alleles in a single cell. The selection of appropriate cells and their use as nuclear donors for SCNT is the most common method for generating edited and genetically modified animals for commercial and research purposes. This article reviews current applications of swine cloning and shares our personal experiences with the procedure in this species. (AU)

• Processo FAPESP: 21/09886-8 - Maturação epigenética do oócito e totipotência: o potencial do oócito e sua relação com o nicho folicular ovariano
• Beneficiário: Lawrence Charles Smith
• Modalidade de apoio: Auxílio à Pesquisa - Programa SPEC
• Processo FAPESP: 23/12977-0 - Manipulação da via Wnt/b-catenina durante a MIV de oócitos suínos visando a produção de clones por TNCS
• Beneficiário: Thais Naomi Goncalves Nesiyama
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs