| Texto completo | |
| Autor(es): |
Souza, Lucas Henrique Bonfim
;
Silva, Bruno Cansancao
;
Pompeo, Jennifer Nunes
;
Gatto, Kaleb Pretto
;
Lourenco, Luciana Bolsoni
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENOME; v. 68, p. 11-pg., 2025-02-01. |
| Resumo | |
The Physalaemus cuvieri-Physalaemus ephippifer species complex is a Neotropical frog group that encompasses seven well- supported major clades. Although very similar morphologically, the five lineages previously karyotyped show notorious cytogenetic signatures. There is also evidence of ancient secondary contact between P. ephippifer, which has heteromorphic sex chromosomes, and the lineage known as L1B, which lacks sex chromosome heteromorphism. Here, to aid comparative analysis within this complex, we mapped the U2 small nuclear RNA (snRNA) gene using fluorescent in situ hybridization (FISH). All samples presented a U2 snRNA gene cluster terminally in the short arm of chromosome 6. Additional small FISH signals were also revealed, particularly in one lineage with previously noted polymorphism of nucleolar organizer regions. Moreover, one additional site contributed for the analysis of sex chromosomes, since the Z chromosome of P. ephippifer harbors a small FISH signal, which is absent in the W chromosome. In lineage L1B, chromosome 9--which is homologous to the sex chromosomes of P. ephippifer--is polymorphic for a small FISH signal, as did the Z chromosome in the group derived from the contact between these lineages. Finally, nucleotide sequence analysis revealed some truncated gene sequences, suggesting the presence of pseudogenes of the U2 snRNA gene in these frogs. (AU) | |
| Processo FAPESP: | 21/00464-3 - O papel dos cromossomos sexuais na divergência de anuros do complexo de espécies Physalaemus cuvieri - Physalaemus ephippifer: explorando as barreiras reprodutivas em uma zona de contato entre linhagens divergentes |
| Beneficiário: | Luciana Bolsoni Lourenço |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Regular |