Autor(es)

Pedroso, Marcio P. ^{[1, 2, 3]} ; Ferreira, Ernesto C. ^{[1, 2]} ; Hantao, Leandro W. ^{[1, 2]} ; Bogusz, Jr., Stanislau ^{[1, 2]} ; Augusto, Fabio ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, Sao Paulo - Brazil

^[2] INCTBio, Sao Paulo - Brazil

^[3] Fed Univ Lavras UFLA, Dept Chem, Lavras, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 34, n. 13, p. 1547-1554, JUL 2011.
• Citações Web of Science: 25

Resumo

Combining qualitative data from the chromatographic structure of 2-D gas chromatography with flame ionization detection (GC x GC-FID) and that from gas chromatography-mass spectrometry (GC/MS) should result in a more accurate assignment of the peak identities than the simple analysis by GC/MS, where coelution of analytes is unavoidable in highly complex samples (rendering spectra unsuitable for qualitative purposes) or for compounds in very low concentrations. Using data from GC x GC-FID combined with GC/MS can reveal coelutions that were not detected by mass spectra deconvolution software. In addition, some compounds can be identified according to the structure of the GC x GC-FID chromatogram. In this article, the volatile fractions of fresh and dehydrated pineapple pulp were evaluated. The extraction of the volatiles was performed by dynamic headspace extraction coupled to solid-phase microextraction (DHS-SPME), a technique appropriate for slurries or solid matrices. Extracted analytes were then analyzed by GC x GC-FID and GC/MS. The results obtained using both techniques were combined to improve compound identifications. (AU)