In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate

Autor(es)

Ferrari, Carolina Ruis ; Kitamoto, Karolyne Sayuri de Araujo ; Pela, Vinicius Taioqui ; Taira, even Akemi ; Araujo, Tamara Teodoro ; Thomassian, Larissa Tercilia Grizzo ; Henrique-silva, Flavio ; Pessan, Juliano Pelim ; Buzalaf, Marilia Afonso Rabelo

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN ORAL RESEARCH; v. 38, p. 10-pg., 2024-01-01.

Resumo

The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A total of 135 bovine enamel specimens were prepared and categorized into nine groups (n/group=15) according to the following treatments: Deionized water; Commercial solution 1.0% TMP; 3.0% TMP; 0.1 mg/mL CaneCPI-5+0.5% TMP; 0.1 mg/mL CaneCPI-5+1.0%TMP; and 0.1 mg/mL CaneCPI-5+3.0%TMP. The specimens were treated with the respective solutions for 2 h, followed by acquired enamel pellicle formation for 2 h and exposure to 0.65% citric acid (CA) for 1 min. These procedures were repeated once a day for three consecutive days. Demineralization was assessed by the percentage change in surface hardness (%CSH) and calcium release into CA, analyzed by the Arsenazo III method. The data were evaluated using Kruskal-Wallis/Dunn's tests. Regarding %CSH, CaneCPI-5+3.0%TMP was the most effective treatment when compared to the CaneCPI-5 group alone. As for calcium release into CA, the CaneCPI-5+0.5% TMP and CaneCPI-5 groups (both with lower calcium release) did not significantly differ from the commercial solution. In conclusion, combination of CaneCPI-5 with TMP enhances the protective potential against initial enamel erosion in vitro. (AU)

• Processo FAPESP: 22/11297-3 - Engenharia de película adquirida para o controle da erosão dentária: avaliação in vitro do potencial protetor de diferentes concentrações do trimetafosfato de sódio associado ou não à canecpi-5
• Beneficiário: Karolyne Sayuri de Araujo Kitamoto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 21/14161-2 - Produção recombinante e avaliação funcional de uma proteína híbrida com potencial de interação com a hidroxiapatita, para proteção contra a cárie e erosão dentária
• Beneficiário: Carolina Ruis Ferrari
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto