Isolation and anti-Xanthomonas citri activity of unguinol produced by Aspergillus unguis CBMAI 2140

Autor(es)

dos Santos, Eduarda Araujo ; Marin, Vitor Rodrigues ; de Angelis, Derlene Atilli ; Ferreira, Henrique ; Sass, Daiane Cristina

• Tipo de documento: Artigo Científico
• Fonte: Letters in Applied Microbiology; v. 78, n. 2, p. 8-pg., 2025-02-25.

Resumo

This research investigated active biomolecules from the endophytic fungus Aspergillus unguis (CBMAI 2140), isolated from Passiflora incarnata leaves, as potential antibacterial agents against Xanthomonas citri subsp. citri, the causative agent of citrus canker. The fungal extract was obtained via liquid-liquid extraction with ethyl acetate. Antibacterial activity was assessed using the Resazurin Microtiter Assay Plate, determining inhibitory concentrations (IC90) and the minimum bactericidal concentration (MBC). The extract displayed microbicidal activity at 1050 mu g ml-(1) and inhibited bacterial growth at 350 mu g ml-(1). Fractionation of the extract via normal-phase column chromatography yielded six fractions, with fraction F11 showing the highest antibacterial potential (MBC: 200 mu g ml-(1)). Nuclear magnetic resonance analysis identified Unguinol as the main compound in F11. Fluorescence microscopy demonstrated rapid permeabilization of the X. citri cell membrane upon exposure to the compound, with significant effects observed after 15-30 min. Although no notable anti-biofilm activity was detected, this study represents the first report of Unguinol's antibacterial activity against Xanthomonas species. These findings highlight its potential for agricultural applications, contributing to sustainable development goal 2. (AU)

• Processo FAPESP: 22/10238-3 - Investigação química e biológica de metabólitos produzidos pelo Aspergillus unguis (CBMAI 2140) para o combate ao cancro cítrico
• Beneficiário: Eduarda Araujo dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 24/11225-8 - Avaliação da presença e retenção de biopesticidas, e dos resíduos de degradação, em folhas, frutos cítricos e solo
• Beneficiário: Vítor Rodrigues Marin
• Modalidade de apoio: Bolsas no Brasil - Programa Fixação de Jovens Doutores
• Processo FAPESP: 18/11747-3 - Avaliação do potencial biotecnológico de micro-organismos na produção de substâncias antibacterianas para uso na agricultura.
• Beneficiário: Daiane Cristina Sass
• Modalidade de apoio: Auxílio à Pesquisa - Regular