| Texto completo | |
| Autor(es): |
Dai, Yitao
;
Lourenzon, Vitor
;
Ioca, Laura P.
;
Al-Smadi, Dua
;
Arnold, Lydia
;
Mcintire, Ian
;
Berlinck, Roberto G. S.
;
Eustaquio, Alessandra S.
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MBIO; v. 16, n. 2, p. 19-pg., 2024-12-27. |
| Resumo | |
Pseudovibrio alpha-Proteobacteria have been repeatedly isolated from marine sponges and proposed to be beneficial to the host. Bacterial motility is known to contribute to host colonization. We have previously identified pseudovibriamides A and B, produced in culture by Pseudovibrio brasiliensis Ab134, and shown that pseudovibriamide A promotes flagellar motility. Pseudovibriamides are encoded in a hybrid nonribosomal peptide synthetase-polyketide synthase gene cluster that also includes several accessory genes. Pseudovibriamide A is a linear heptapeptide and pseudovibriamide B is a nonadepsipeptide derived from pseudovibriamide A. Here, we define the borders of the pseudovibriamides gene cluster, assign function to biosynthetic genes using reverse genetics, and test the hypothesis that pseudovibriamides impact motility by modulating gene transcription. RNA-sequencing transcriptomic analyses of strains having different compositions of pseudovibriamides suggested that both pseudovibriamides A and B affect genes potentially involved in motility, and that a compensatory mechanism is at play in mutants that produce only pseudovibriamide A, resulting in comparable flagellar motility as the wild type. The data gathered suggest that pseudovibriamides A and B have opposite roles in modulating a subset of genes, with pseudovibriamide B having a primary effect in gene activation, and pseudovibriamide A on inhibition. Finally, we observed many differentially expressed genes (up to 29% of the total gene number) indicating that pseudovibriamides have a global effect on transcription that goes beyond motility. (AU) | |
| Processo FAPESP: | 18/10742-8 - A função de um peptídeo não-ribossimal para promover a motilidade de Pseudovibrio brasiliensis Ab134 e bactérias relacionadas |
| Beneficiário: | Laura Pavan Ióca |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 16/05133-7 - Estudo do metabolismo de micro-organismos do ambiente marinho: perfil químico e biossíntese |
| Beneficiário: | Laura Pavan Ióca |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |