| Texto completo | |
| Autor(es): |
Astolfi, Bianca Soares
;
Bessas, Naiara Cristina
;
Graminha, Angelica Ellen
;
Becceneri, Amanda Blanque
;
da Silva, Roberto Santana
;
de Lima, Renata Galvao
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Fluorescence; v. 34, n. 6, p. 13-pg., 2023-11-10. |
| Resumo | |
Carbon dots (CDs) exhibit luminescence, biocompatibility, and higher water solubility. This material has been developed for biological applications, specifically in bioimaging. In this work, the gelatin carbon dots (CDg) was obtained from commercial gelatin using a hydrothermal method in domestic microwave, and the suppression fluorescent mechanism were enhanced by the addition of the [Ru-II(bdq)(NO)(tpy)](3+) (Rubdq-NO+) complex ion. After purification through a dialysis bag, the resulting CDs (CDg) exhibit fluorescent emission at 400 nm and maintained fluorescence stability in an aqueous solution (pH=7) for 30 days under 5 degrees C. Fluorescence quenching studies revealed an electrostatic interaction between the negative charge from CDg (delta=-20 mV) and the positively charged nitrosyl (NO+) ligand of the ruthenium complex (Rubdq-NO+), resulting in quenching of the CDg fluorescence due to the inner filter effects (IFE). The chemiluminescence reaction of CDg and Rubdq-NO-CDg in presence of norepinephrine (NOR) were evaluated. NOR in PBS are liable to undergo spontaneous oxidation to quinone form (NOR-quinone). CDg are believed interact with NOR-quinone and an electron transfer occur obtained CDg+ accompanied to green emission fluorescence (520 nm). While for Rubdq-NO-CDg in presence of NOR, the green emission occurs accompanied by NO0 release using DAF-2 probe. (AU) | |
| Processo FAPESP: | 20/03367-6 - Fototerapia utilizando complexos rutênio-ftalocianinas como geradores de espécies reativas de oxigênio e nitrogênio: estudo do mecanismo de ação in vitro em diferentes modelos 2D e 3D de células cancerígenas |
| Beneficiário: | Amanda Blanque Becceneri |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |