Autor(es)

Monteiro, Naara Gabriela ; de Oliveira-Filho, Odir Nunes ; Gandolfo, Maria Isabela Lopes ; da Silva, Ana Claudia Ervolino ; Pitol-Palin, Leticia ; Botacin, Paulo Roberto ; Mulinari-Santos, Gabriel ; Batista, Fabio Roberto de Souza ; Okamoto, Roberta

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGY-BASEL; v. 14, n. 2, p. 16-pg., 2025-02-01.

Resumo

Simple Summary: This study explored the impact of Rubus coreanus (RC) on peri-implant bone healing in rats with and without estrogen deficiency. The rats were divided into four groups: healthy (SHAM), healthy with RC treatment (SHAM/RC), ovariectomized (OVX), and ovariectomized with RC treatment (OVX/RC). RC was given 30 days after ovariectomy, with implant placement occurring 90 days later. The results showed that RC treatment improved bone healing, with the SHAM/RC group exhibiting the highest bone biomineralization and removal torque. RC also helped balance bone formation and resorption in the ovariectomized rats. These findings suggest that RC can enhance peri-implant bone healing in both healthy and ovariectomized rats, with hormonal status influencing its effectiveness. Further research is needed to investigate the mechanisms. Estrogen deficiency contributes to osteoporosis and can therefore compromise the peri-implant bone. Hence, this study evaluated peri-implant bone healing when Rubus coreanus (RC) was administered orally in ovariectomized and healthy rats. Forty 4-month-old female rats were divided into four groups: SHAM (healthy rats), SHAM/RC (healthy rats treated with RC), OVX (ovariectomized rats), and OVX/RC (ovariectomized rats treated with RC). The oral administration of RC started thirty days after ovariectomy, and implant placement into the rat tibia occurred ninety days after the ovariectomy. Euthanasia occurred sixty days after implantation. The analyses performed included removal torque, RT-PCR, confocal microscopy, and immunolabeling. A significance level of p < 0.05 was considered for all tests. The highest reverse torque values were observed in the SHAM/RC group, followed by the OVX/RC group. Confocal microscopy showed the greatest bone biomineralization in the SHAM/RC group, followed by the OVX/RC group. RT-PCR data indicated that RC decreased the RANKL/OPG ratio in both conditions. Immunohistochemistry demonstrated a balance between bone formation and resorption in all groups, especially stimulating osteoblastogenesis in both treated groups. In conclusion, RC enhanced peri-implant bone healing and biomineralization in both healthy and ovariectomized rats, with stronger effects in healthy rats, suggesting that estrogen may enhance its efficacy. These findings support RC's potential as a prophylactic and therapeutic agent. (AU)

• Processo FAPESP: 21/12692-0 - Rubus coreanos na funcionalização da superfície de implantes: impacto sobre o reparo periimplantar de ratas com deficiência de estrógeno
• Beneficiário: Odir Nunes de Oliveira Filho
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/02798-3 - Avaliação das respostas de biomineralização pelo Rubus coreanus frente à deficiência de estrógeno: um estudo translacional
• Beneficiário: Naara Gabriela Monteiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/08617-5 - Avaliação de proteínas da matriz extracelular durante o processo de reparo na interface osso/implante de ratas ovariectomizadas e tratadas com genisteína: análise microtomográfica e por imunoistoquímica
• Beneficiário: Naara Gabriela Monteiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica