Enhancing biosensor capabilities through laccase immobilization in nanostructured Langmuir-Blodgett films for phenol detection

Autor(es)

Machado, A. C. ; Salvo-Comino, C. ; Rodriguez-Mendez, M. L. ; Caseli, L.

• Tipo de documento: Artigo Científico
• Fonte: RESULTS IN SURFACES AND INTERFACES; v. 18, p. 9-pg., 2024-11-27.

Resumo

Fabricating nanoscale structures combining various elements with specific functionalities is a pertinent strategy for enhancing sensor capabilities. In this study, we immobilized Laccase (Lac) in a biomimetic environment provided by a nanostructured Langmuir-Blodgett (LB) film of an amphiphilic material (octadecylamine, ODA), where poly(3-hexylthiophene-2,5-diyl) (P3HT) has been coimmobilized as the electron mediator. Tensiometry demonstrates that the enzyme is incorporated in the floating monolayers efficiently. The monolayers were transferred to solid substrates as LB films and characterized using UV-vis and infrared spectroscopies and Atomic Force Microscopy, exhibiting a quasi-ordered structure with low roughness. The films' ability to detect phenols was evaluated through enzymatic activity estimation using colorimetric and voltammetry measurements against vanillin, catechol, and pyrogallol. Our results demonstrate that the electrochemical response depends on the phenol's nature, with limits of detection for vanillin, catechol, and pyrogallol at 1.10.10(-6), 2.13.10(-7), and 2.07.10(-7) mol.L-1, respectively. Additionally, the intensity of the response does not correlate with film thickness, confirming that the electrochemical reaction is a surface phenomenon for catechol and pyrogallol. We believe this study provides valuable insights into phenol detection in the food industry, which is crucial for ensuring food safety, quality, and authenticity by aiding in regulatory compliance, process optimization, and product preservation. (AU)

• Processo FAPESP: 22/03736-7 - Estudos sobre a interação de compostos bioativos em modelos de biointerface e construção de dispositivos nanoestruturados bioinspirados
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/04241-1 - Filmes de Langmuir-Blodgett da enzima lacase para detecção de derivados fenólicos
• Beneficiário: André Campos Machado
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 23/00329-4 - Desenvolvimento de biossensores para compostos fenólicos utilizando filmes de Langmuir e Langmuir-Blodgett contendo lacase
• Beneficiário: André Campos Machado
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado