Expression of MHC I Isoforms in Bovine Placentomes: Impact of Cloning

Autor(es)

Barreto, Rodrigo da Silva Nunes ; Mancanares, Ana Carolina Furlanetto ; Miglino, Maria Angelica ; Meirelles, Flavio Vieira ; Oliveira, Lilian de Jesus

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY SCIENCES; v. 12, n. 3, p. 13-pg., 2025-02-21.

Resumo

Major histocompatibility complex class I (MHC-I) gene expression in the placenta is modulated to tailor the maternal immune response to fetal antigens during pregnancy. This study evaluated MHC-I expression through immunohistochemistry (IHC) using an anti-mouse preimplantation embryo development (PED) clone Qa-2 and anti-bovine leukocyte antigen I (BoLA) monoclonal antibody clone IL-A88 (n = 23), as well as RT-qPCR (n = 17) for classical and non-classical (BoLA-NC) genes in control and cloned bovine placentomes during early and near-term gestation. Control samples showed minimal Qa-2 protein expression in early gestation, with intense labeling in trophoblasts and the maternal uterine epithelium near term. In contrast, cloned samples exhibited intense Qa-2 labeling in both maternal and trophoblastic epithelia at both stages, while trophoblast giant cells (TGCs), located apposed to the maternal epithelium, showed no labeling. Control samples exhibited intense IL-A88 labeling in the maternal epithelium at both stages. In cloned samples, weak to no labeling was observed in early gestation, with intense labeling in maternal and fetal epithelium near term. RT-qPCR revealed significant upregulation of BoLA-NC3 in early gestation, with sustained elevated expression in cloned samples in the near term. These findings suggest that altered BoLA protein expression and gene regulation in cloned pregnancies may contribute to pregnancy complications and increased losses. (AU)

• Processo FAPESP: 09/06702-1 - Modelo de estudo de imunomodulação em bovinos: Expressão do MHC classe 1b em células trofoblásticas e mesênquimais da placenta de conceptos clonados e naturais
• Beneficiário: Rodrigo da Silva Nunes Barreto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 21/05445-7 - Fábrica de tecidos funcionalizados: bioengenharia baseada nas interações da matriz extracelular com biopolímeros e bioimpressão
• Beneficiário: Maria Angelica Miglino
• Modalidade de apoio: Auxílio à Pesquisa - Temático