Antimicrobial Effect of Reactive Iodine Species Produced by Photodynamic Therapy Against Streptococcuspneumoniae in a Lung Alveolar Transwell Model

Autor(es)

Jasinevicius, Gabriel Oliveira ; Guimaraes, Francisco Eduardo Gontijo ; Kurachi, Cristina

• Tipo de documento: Artigo Científico
• Fonte: 2024 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, SBFOTON IOPC 2024; v. N/A, p. 3-pg., 2024-01-01.

Resumo

Antimicrobial photodynamic therapy (aPDT) effectiveness against pathogenic microorganisms can be enhanced by potassium iodide (KI). KI achieves this by forming longer-lived reactive iodine species (RIS), compared to the reactive oxygen species (ROS) in conventional PDT. This study investigated the synergistic effects of KI and Methylene Blue (MB) in aPDT against Streptococcus pneumoniae within a lung alveolar transwell model. Results showed that KI significantly enhanced MB-mediated aPDT, increasing efficacy against S. pneumoniae colony-forming units (CFUs). Notably, the photoproducts (RIS) alone reduced S. pneumoniae CFUs by over 3 logs without harming mammalian cells. This highlights the potential of KI-enhanced aPDT. (AU)

• Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático