| Texto completo | |
| Autor(es): Mostrar menos - |
Pedrozo-Penafiel, Marlin
;
Gutierrez-Beleno, Luis
;
Mendoza, Cesar A. D.
;
Freire-Junior, Fernando L.
;
Lima, Mauro A.
;
Teixeira, Tamara
;
Rocha, Fillipe V.
;
Zolin, Gabriela V. S.
;
Garrido, Saulo S.
;
Lazzarini, Ana B.
;
Netto, Adelino V. G.
;
Haddad, Felipe F.
;
Joao, Emilio E.
;
Santos, Jean L.
;
Scarim, Caue B.
;
Farias, Renan L.
;
Aucelio, Ricardo Q.
Número total de Autores: 17
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS OMEGA; v. N/A, p. 13-pg., 2025-04-15. |
| Resumo | |
This study explores the biological effects and imaging potential of graphene quantum dots (GQDs) and their nitrogen-doped, glutathione-modified form (GQDs-GSH) in human cell models. We evaluated the cell viability, hemolysis potential, and irritation effects of these nanomaterials, showing that both GQDs and GQDs-GSH maintained 100% cell viability in the range of 16.25-260 mu g mL(-1), caused minimal RBC hemolysis (<9%), and presented low irritation scores in HET-CAM assays. Interaction studies revealed distinct binding behaviors with biomolecules: GQDs used a static quenching mechanism with human serum albumin (HSA), while GQDs-GSH exhibited a mixed static and dynamic interaction. Additionally, GQDs-GSH exhibited a stronger affinity for calf thymus DNA (ctDNA) without disturbing the DNA secondary structure. Fluorescence microscopy-based apoptotic cell staining assays indicated the adherence potential of both GQDs and GQDs-GSH. GQDs tended to accumulate more prominently in regions associated with apoptotic bodies, whereas GQDs-GSH tended to localize on the membranes of spheroid-shaped cells. (AU) | |
| Processo FAPESP: | 22/14041-0 - Planejamento e investigação do potencial antitumoral de complexos metálicos contra células metastáticas e/ou quimiorresistentes |
| Beneficiário: | Adelino Vieira de Godoy Netto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |