| Texto completo | |
| Autor(es): |
Lopes-Goncalves, Guilherme
;
Costa-Pessoa, Juliana Martins
;
de Ponte, Mariana Charleaux
;
Braz, Heitor Macedo
;
Oliveira-Souza, Maria
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN PHYSIOLOGY; v. 16, p. 14-pg., 2025-03-19. |
| Resumo | |
Introduction Acute kidney injury (AKI) is a clinical syndrome characterized by rapid decline in renal function with varying severity. In this context, tubular function is impaired in ischemia-induced AKI. Although there are no effective therapies for AKI, many compounds have been reported to reduce kidney injury, such as apelin-13. Considering the relevance of proximal tubular cells in maintaining fluid and electrolyte homeostasis, the effects of apelin-13 on tubular injury or sodium proximal transport remain unclear. Thus, the present study aims to evaluate the effects of exogenous administration of apelin-13 in the renal ischemia/reperfusion (I/R) model, with particular focus on renal function, injury markers, and tubular proliferation.Methods Male C57BL/6 mice were initially treated with a vehicle or high dose of apelin-13 (200 mu g/kg/day) and subjected to kidney bilateral ischemia procedure for 30 min or a sham surgery. The mice were euthanized by exsanguination 2 d after the ischemic procedure. Then, the renal function was assessed through the plasma urea level and creatinine clearance. Tubular injury was evaluated by hematoxylin and eosin staining. Kidney injury molecule 1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), megalin, Ki67, and phospho ERK 1/2 (Thr202/Tyr204) were evaluated through immunohistochemical or immunoblotting experiments. Moreover, the murine proximal tubular cells (TKPTS) were treated with apelin-13 (100 nM) to evaluate the activity of the Na+/H+ exchanger isoform 3 (NHE3) via intracellular pH measurements.Results Initial administration of apelin-13 did not improve tubular injury, creatinine clearance, or plasma urea level after renal I/R. Moreover, KIM-1 and NGAL markers were markedly increased after renal I/R and were not reduced in the apelin-13 + I/R group. Furthermore, megalin downregulation by renal I/R was not prevented by apelin-13. Interestingly, apelin-13 worsened the renal responses to tubular proliferation after renal I/R as Ki67 and phosphorylation of ERK/1/2 (Thr202/Tyr204) were sharply reduced in the apelin-13 + I/R group. In vitro experiments also demonstrated that apelin-13 inhibited NHE3 activity in murine proximal tubular cells.Conclusion The overall findings suggest that apelin-13 suppresses tubular proliferation and potentially impairs the adaptive response to renal I/R injury, thereby highlighting its relevance in ischemia-induced AKI. (AU) | |
| Processo FAPESP: | 22/01288-7 - Fatores envolvidos na progressão da lesão renal induzida por nefropatia cristalina ou isquemia/reperfusão: estudos in vivo e in vitro |
| Beneficiário: | Maria Oliveira de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/05371-6 - Implicações da sinalização apelinérgica sobre o transporte renal de sódio e água: papel da isquemia/reperfusão |
| Beneficiário: | Guilherme Lopes Gonçalves |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |