Interaction of Myrsinoic acid a with biomembrane models: Differential effects on DPPC and DPPS properties revealed by surface rheology and vibrational spectroscopy

Autor(es)

dos Santos, Ana Gabrieli A. ; Cassas, Fernando ; dos Santos, Kevin Figueiredo ; de Medeiros, Livia Soman ; Veiga, Thiago Andre Moura ; Caseli, Luciano

• Tipo de documento: Artigo Científico
• Fonte: Biophysical Chemistry; v. 322, p. 8-pg., 2025-07-01.

Resumo

This study investigates the interactions of Myrsinoic acid A, a natural compound with reported anti-inflammatory and antitumor properties, with lipid monolayers composed of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylserine (DPPS). Utilizing tensiometry, polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS), Brewster Angle Microscopy (BAM), and surface rheology, we analyzed how Myrsinoic acid A affects the structural and mechanical properties of these lipid systems. The PM-IRRAS spectra revealed that Myrsinoic acid A induced disorder in the DPPC monolayer, shifting CHI asymmetric stretching peaks and decreasing packing order, while DPPS remained structurally stable. Surface rheology measurements showed reduced elasticity in both lipids, with differential effects on viscosity: a decrease for DPPC and an increase for DPPS, indicating varied molecular interactions. BAM images confirmed that DPPC maintained a homogeneous morphology, while DPPS displayed aggregate formation, suggesting distinct lipid-drug interactions. These findings highlight the importance of lipid composition in modulating the effects of Myrsinoic acid A on membrane properties, providing insights into its potential therapeutic applications in targeting tumorigenic versus non-tumorigenic cells. (AU)

• Processo FAPESP: 22/03736-7 - Estudos sobre a interação de compostos bioativos em modelos de biointerface e construção de dispositivos nanoestruturados bioinspirados
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/04095-0 - Abordagens analíticas para a descoberta de compostos citotóxicos desconhecidos a partir de plantas
• Beneficiário: Thiago André Moura Veiga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/13811-9 - Estudo da interação entre o envelope do SARS-CoV-2 e a membrana da célula hospedeira usando modelos miméticos
• Beneficiário: Kevin Figueiredo dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado