| Texto completo | |
| Autor(es): |
Peralta-Martinez, Ramon
;
Visentin, Araceli
;
Salgueiro, Mariano
;
Borkosky, Silvia Susana
;
Aleixo, Mariana Araujo Ajalla
;
Portugal, Rodrigo Villares
;
Sanchez, Ignacio Enrique
;
Prat-Gay, Gonzalo
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Protein Science; v. 34, n. 6, p. 14-pg., 2025-06-01. |
| Resumo | |
Protein-RNA phase separation is at the center of membraneless biomolecular condensates governing cell physiology and pathology. Using an archetypical viral protein-RNA condensation model, we determined the sequence of events that starts with sub-second formation of a protomer with two RNAs per protein dimer. Association of additional RNA molecules to weaker secondary binding sites in this protomer kickstarts crystallization-like assembly of a molecular condensate. Primary nucleation is faster than the sum of secondary nucleation and growth, which is a multistep process. Protein-RNA nuclei grow over hundreds of seconds into filaments and subsequently into nanoclusters with approximately 600 nm diameter. Cryoelectron microscopy reveals an internal structure formed by incoming layers of protein-RNA filaments made of ribonucleoprotein oligomers, reminiscent of genome packing of a nucleocapsid. These nanoclusters progress to liquid condensate droplets that undergo further partial coalescence to yield typical hydrogel-like protein-RNA coacervates that may represent the scaffold of large viral factory condensates in infected cells. Our integrated experimental kinetic investigation exposes rate-limiting steps and structures along a key biological multistep pathway present across life kingdoms. (AU) | |
| Processo FAPESP: | 20/06062-1 - Complexos biológicos em ação, in vitro e in silício (Explorando Big-Data em criomicroscopia eletrônica) |
| Beneficiário: | Marin Gerard van Heel |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 22/05088-2 - Ampliando o impacto de infraestruturas de pesquisa com uma perspectiva global: um modelo de centros regionais |
| Beneficiário: | Rodrigo Villares Portugal |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |