Modulating the activity of the APC/C regulator SISAMBA improves the sugar and antioxidant content of tomato fruits

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de Oliveira, Perla Novais ; de Souza, Leonardo Perez ; Ferreira, Pedro Boscariol ; Mauxion, Jean Phillippe ; da Silva, Luis Felipe Correa ; Chang, Ana Isabela ; Saleme, Marina de Lyra Soriano ; Zhu, Feng ; Garcia, Sara Selma ; De Beukelaer, Herman ; Martins, Marina C. M. ; Rosado-Souza, Laise ; Pino, Lilian Ellen ; Bollier, Norbert ; Peres, Lazaro Eustaquio Pereira ; Vandepoele, Klaas ; Goossens, Alain ; Gonzalez, Nathalie ; Fernie, Alisdair R. ; Eloy, Nubia Barbosa

• Tipo de documento: Artigo Científico
• Fonte: Plant Biotechnology Journal; v. N/A, p. 21-pg., 2025-06-08.

Resumo

The Anaphase-Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase that plays a crucial role in ubiquitin-dependent proteolysis of key cell cycle regulators, which is completed by the 26S proteasome. Previously, SAMBA, a plant-specific regulator of the APC/C, was identified in Arabidopsis as a critical factor controlling organ size through the regulation of cell proliferation. Here, by assessing its role in the crop tomato (Solanum lycopersicum), we confirm that SAMBA is a conserved APC/C regulator in plants and shows additional roles, including the modulation of fruit shape and changes in sugar metabolism. Two slsamba genome-edited lines were produced and characterized, and showed delayed growth, reduced plant size, and altered fruit morphology, which were linked to changes in cell division and expansion. Notably, untargeted metabolomics revealed altered flavonoid profiles, along with elevated Brix values in the fruits, indicating a sweeter taste. Accordingly, transcriptomics uncovered a change in temporal gene expression gradients during early fruit development, correlating with the alterations in sugar metabolism and revealing changes in cell wall biosynthesis genes. This study provides the first evidence of SAMBA's role in regulating fruit development, metabolic content, and ultimately, quality. These important findings offer potential applications for improving the nutritional quality and overall performance of tomatoes. (AU)

• Processo FAPESP: 23/13107-0 - Caracterização do efeito da superexpressão do gene SAMBA em tomateiro Micro-Tom
• Beneficiário: Ana Isabela Chang
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 21/06611-8 - Uso das subunidades do APC para o estudo da regulação de pequenas moléculas no ciclo celular
• Beneficiário: Perla Novais de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/03212-5 - Caracterização funcional da subunidade 5 do Complexo Promotor da Anáfase
• Beneficiário: Luís Felipe Correa da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/10333-8 - Uso das subunidades do APC para o estudo da regulação de pequenas moléculas no ciclo celular
• Beneficiário: Nubia Barbosa Eloy
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 20/00888-5 - Uso das subunidades do APC para o estudo da regulação de pequenas moléculas no ciclo celular
• Beneficiário: Marina de Lyra Soriano Saleme
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado