Autor(es)

Steva, Caroline Gennari ; Dominguete, Matheus Henrique Lopes ; Moraes, Paulo de Camargo ; Montalli, Victor Angelo Martins ; de Freitas, Andre Luis Santana ; Sperandio, Marcelo

• Tipo de documento: Artigo Científico
• Fonte: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 139, n. 6, p. 10-pg., 2025-04-24.

Resumo

Objective. The objective of this paper was to compare the accuracy of predicting malignant transformation of oral leukoplakia using the 585-620 nm detector when compared with data obtained with the 661-683 nm detector. Study Design. This was a single-factor retrospective longitudinal experimental study, whose factor will be DNA content (DNA ploidy) at 4 levels, G1 phase, S phase, mitosis phase (G2), and DNA excess (4cER). The sample is a cohort of individuals diagnosed with oral leukoplakia (N = 97) where 18 cases evolved into cancer and 79 did not. Results. The DNA content parameters 4cER, G2, and G1/G2 ratios were associated with malignant transformation (P < .05). Two analog algorithms of these findings generated positive predictive values (PPVs) of 38% (67% sensitivity and 76% specificity) and 40% (61% sensitivity and 80% specificity). DNA content by flow cytometry can achieve a PPV of 40% in high-risk lesions and negative predictive value (NPV) of 90% in low-risk lesions. Conclusions. There was no significant difference among the percentages of nuclei in the G1, S, G2, and excess G2 (4cER) phases comparing the 661-683 nm and 585-620 nm detectors. The 585-620 nm detector achieved PPV and NPV equivalent to those observed with the 661-683 nm detector. (Oral Surg Oral Med Oral Pathol Oral Radiol 2025;139:729-738) (AU)

• Processo FAPESP: 17/06579-1 - Valor preditivo de ploidia de DNA combinada a marcardores de transformação maligna em lesles orais cancerizáveis
• Beneficiário: Marcelo Sperandio
• Modalidade de apoio: Auxílio à Pesquisa - Regular