| Texto completo | |
| Autor(es): |
de Lima, Lucas F.
;
Estrela, Paulo Felipe Neves
;
Faustino, Lucas Costa
;
Souza, Jonatas, V
;
Geroncio, Everson T. S.
;
de Lima, Leandro Fonseca
;
Bailao, Elisa Flavia Luiz Cardoso
;
de Araujo, William R.
;
Paixao, Thiago R. L. C.
;
Duarte, Gabriela R. M.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Analytica Chimica Acta; v. 1372, p. 9-pg., 2025-10-22. |
| Resumo | |
Background: The increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA), particularly due to the presence of the mecA gene, emphasizes the need for decentralized, rapid, and accurate molecular diagnostics. While qPCR remains the gold standard method, its dependence on expensive equipment and centralized labs limits accessibility in field or point-of-care (POC) settings. To address this limitation, we developed an Electrochemical Loop-Mediated Isothermal Amplification (E-LAMP) platform for rapid, low-cost, and highly sensitive detection of the mecA gene, using 3D-printed electrodes and a smartphone-controlled potentiostat. Results: The system couples LAMP with electrochemical detection via cresol red, a pH-sensitive dye that undergoes a peak potential shift upon proton release during DNA amplification. Linear sweep voltammetry (LSV) was used to monitor these changes, enabling quantitative analysis. The platform achieved a limit of detection (LOD) of 11 copies mu L- 1, which is 30 times more sensitive than conventional colorimetric LAMP. The device submitted to UV-laser surface treatment, enhancing electrode conductivity and response reproducibility. The assay produced results in under 31 min and showed 100 % agreement with qPCR across 10 real MRSA-positive and negative samples, with no cross-reactivity toward non-target bacteria. Quantification of environmental limited settings. This method shows high promise for wider application in infectious disease surveillance, (AU) | |
| Processo FAPESP: | 23/00246-1 - Dispositivos miniaturizados visando à produção em larga escala: fabricação, caracterização e aplicações in-situ |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 23/12589-0 - Fabricação de um arranjo de (bio)sensores eletroquímicos por gravação a laser de CO2 visando o desenvolvimento de dispositivo wearable |
| Beneficiário: | Lucas Felipe de Lima |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 22/03250-7 - Desenvolvimento de biossensores portáteis e acessíveis para diagnósticos clínicos de infecções bacterianas e virais |
| Beneficiário: | William Reis de Araujo |
| Modalidade de apoio: | Auxílio à Pesquisa - Projeto Inicial |