Autor(es)

Takayasu, Bianca S. ; Martins, Igor R. ; Uemi, Miriam ; Onuki, Janice ; Machado-Santelli, Glaucia M.

• Tipo de documento: Artigo Científico
• Fonte: BIOMOLECULES; v. 15, n. 7, p. 17-pg., 2025-06-27.

Resumo

Sitosterol (Sito) is a phytosterol with bioactive properties, including reducing atherosclerosis risk and anti-inflammatory and antitumoral effects. However, it can be oxidized by reactive oxygen species such as ozone (O3), producing oxyphytosterols with harmful effects such as cytotoxicity, oxidative stress, and proatherogenicity. Ozone, a strong oxidant and common pollutant, can alter plant steroid compounds, raising concerns about dietary oxyphytosterol intake. Studies identify beta-Secosterol (beta Sec) as the primary ozone-derived oxyphytosterol from Sito, exhibiting cytotoxic effects on HepG2 human liver tumor cells. This study investigated beta Sec's biological effects on two rat liver cell lines: BRL-3A (immortalized) and HTC (tumoral), examining cell death, cell cycle progression, morphology, and cytoskeleton organization. While Sito influenced cell metabolic activity without affecting cell survival or morphology, beta Sec demonstrated significant cytotoxicity in both cell lines. It induced G0/G1 cell cycle arrest and disrupted cytoskeleton organization, with different implications: BRL-3A cells showed persistent cytoskeletal changes potentially linked to tumor induction, while HTC cells displayed chemoresistance, restoring cytoskeletal integrity and enhancing metastatic potential. These findings reveal beta Sec's complex, context-dependent effects, suggesting it may promote tumor-like behavior in non-tumoral cells and resistance mechanisms in cancer cells, contributing to understanding oxyphytosterols' implications for physiological and pathological conditions. (AU)

• Processo FAPESP: 15/17177-6 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 14/14801-8 - Oxisteróis são capazes de modificar a ubiquitina e comprometer o processo proteolítico ubiquitina proteassoma?
• Beneficiário: Miriam Uemi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/19506-8 - 7th International Conference on Food Factors (ICoFF2019) & the 12th International Conference and Exhibition on Nutraceuticals and Functional Foods (ISNFF2019)
• Beneficiário: Janice Onuki
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior