| Texto completo | |
| Autor(es): |
Ejarque, Julia B.
;
Couto, Anna C. F.
;
Matos, Thabata
;
Duarte, Evandro L.
;
Lamy, M. Teresa
;
Rozenfeld, Julio H. K.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biophysical Chemistry; v. 326, p. 8-pg., 2025-08-04. |
| Resumo | |
GD3 is a disialoganglioside overexpressed in several types of cancer cells. The synthetic cationic lipid DODAB has been successfully employed as a vaccine adjuvant, and would be suitable to enhance GD3 immunogenicity. Here, mixed dispersions of GD3 and DODAB were characterized by Differential Scanning Calorimetry (DSC) and Electron Paramagnetic Resonance (EPR) spectroscopy. GD3 is miscible with DODAB, and decreases the DODAB gel-fluid transition cooperativity. GD3 does not affect the temperature hysteresis between gel-fluid and fluid-gel transitions. GD3 does not affect the formation of a subgel phase in DODAB bilayers cooled below 15 degrees C. GD3 decreases the acyl chain packing of the DODAB subgel phase, which could explain the broad and shallow exothermic event between 5 degrees C and 20 degrees C that appears on thermograms of mixed dispersions. These results might contribute to the development of novel GD3-based cancer immunotherapies, including at the low temperatures involved in cold chain stability. (AU) | |
| Processo FAPESP: | 19/17207-3 - Caracterização estrutural de bicamadas catiônicas contendo disialogangliosídeo GD3 |
| Beneficiário: | Anna Clara de Freitas Couto |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 14/50983-3 - INCT 2014: fluidos complexos |
| Beneficiário: | Antonio Martins Figueiredo Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/19077-1 - Desenvolvimento de novos adjuvantes de vacinas capazes de ativar células T CD1d-restritas |
| Beneficiário: | Julio Henrique Kravcuks Rozenfeld |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/13368-4 - Sistemas nanoestruturados: de modelos biomiméticos de membranas a carreadores de bioativos |
| Beneficiário: | Karin Do Amaral Riske |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |