Autor(es)

da Silva, Daniel Jose ; Mendes Cobe, Gabriella ; Colonese Vlasman, Raphael ; Catalani, Luiz Henrique

• Tipo de documento: Artigo Científico
• Fonte: ACS POLYMERS AU; v. N/A, p. 12-pg., 2025-10-22.

Resumo

Polypeptides are attractive, renewable, and biocompatible materials for broad potential biomedical, pharmaceutical, and regenerative medicine applications. In this study, star-shaped 3-arm amphiphilic polypeptides with self-assembly characteristics were synthesized by ring-opening polymerization (ROP) of N-carboxyanhydride (NCA), incorporating L-leucine and L-lysine to form a core-shell structure. These polymers were thoroughly characterized through various techniques, revealing that their rheological behavior and self-assembly are influenced by factors such as the length of the star-shaped arms, the proportion of hydrophobic amino acids, and the surrounding pH. The synthesized polypeptides can self-assemble into five distinct secondary structures, mimicking natural proteins. Our findings evidence that the design of the block size of the hydrophilic L-lysine core and hydrophobic L-leucine shell shapes the ability to form a physical hydrogel, exhibiting shear-thinning rheological characteristics and a rapid mechanical response. The internal microstructure of the hydrogel is based on a supramolecular self-assembly structure consisting of highly connected nanofibrils. (AU)

• Processo FAPESP: 20/15986-2 - Síntese e caracterização de polipeptídeos funcionalizáveis e fotoreticuláveis para aplicação em impressão 3D
• Beneficiário: Raphael Colonese Vlasman
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/13492-2 - Arcabouços sintéticos e naturais aplicados à medicina regenerativa
• Beneficiário: Luiz Henrique Catalani
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/16427-2 - Polipeptídeos derivados de N-carboxianidrido fotorreticuláveis com características pseudoplásticas para impressão 3D baseada em extrusão
• Beneficiário: Daniel José da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado