| Texto completo | |
| Autor(es): |
Hidalgo, Marco Antonio Rimachi
;
Caldeira, Francois Isnaldo Dias
;
Orrico, Silvana Regina Perez
;
Leite, Fabio Renato Manzolli
;
Scarel-Caminaga, Raquel Mantuaneli
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMEDICINES; v. 13, n. 8, p. 21-pg., 2025-08-20. |
| Resumo | |
Aim: Peroxisome Proliferator-Activated Receptor Gamma (PPAR-gamma) plays important anti-inflammatory roles, including in periodontitis. This systematic review with a meta-analysis compiles evidence on the transcriptional and translational levels of PPAR-gamma in clinical and experimental periodontitis studies, alongside functional enrichment and PPAR-gamma interaction network analyses. Method: Electronic searches were conducted in six databases for publications up to February 2024. For the meta-analysis of experimental studies of periodontitis, PPAR-gamma levels in the periodontal tissues were assessed through gene expression (RT-qPCR) or protein expression (Western blotting). In the clinical periodontitis studies, PPAR-gamma levels in the gingival tissues were evaluated through protein expression (immunohistochemistry). A risk of bias (RoB) assessment was performed using the Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) and Newcastle Ottawa Scale (NOS) tools for experimental and clinical studies, respectively. The enrichment analysis was performed using the g:Profiler tool, and gene interaction networks were analyzed using GeneMANIA. Results: The meta-analysis demonstrated significantly lower PPAR-gamma protein levels in the periodontal tissues from animals with periodontitis. PPARG mRNA and PPAR-gamma quantification through immunohistochemistry remained inconclusive. The bioinformatics analyses indicated direct or indirect PPAR-gamma-associated molecules involved in the immune response to periodontitis. The PPAR-gamma protein expression was higher in periodontal tissues from healthy animals compared to that from those with periodontitis. Conclusions: Given the inconclusive findings of RT-qPCR and immunohistochemistry, further PPARG mRNA and PPAR-gamma protein evaluations are needed to clarify their levels in the periodontal tissues. (AU) | |
| Processo FAPESP: | 20/12788-5 - Avaliação Morfo-funcional do PPARG e IL7 como moléculas-chave para alterações glicêmicas e imunológicas do Diabetes Mellitus tipo 2 e periodontite |
| Beneficiário: | Raquel Mantuaneli Scarel Caminaga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/11639-6 - Influência do PPARG em alterações glicêmicas e imunológicas do Diabetes Mellitus Tipo 2 e Periodontite |
| Beneficiário: | Marco Antonio Rimachi Hidalgo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 22/11382-0 - Investigação de IRS-2 e SOD-1 como biomarcadores da homeostase do metabolismo glicêmico e oxidativo em pacientes com diabetes mellitus tipo 2 e periodontite submetidos a tratamento periodontal |
| Beneficiário: | François Isnaldo Dias Caldeira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |