| Texto completo | |
| Autor(es): |
Bellini, Amanda
;
Dias, Luana Mendonca
;
Abreu-Pereira, Cesar Augusto
;
Gorayb-Pereira, Ana Luiza
;
Klein, Marlise Inez
;
Pavarina, Ana Claudia
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Photodiagnosis and Photodynamic Therapy; v. 56, p. 13-pg., 2025-12-01. |
| Resumo | |
Objective To evaluate whether pretreatment strategies targeting the extracellular matrix (ECM), such as DNase I and low-frequency ultrasound, enhance the efficacy of successive antimicrobial photodynamic therapy (aPDT) against Candida albicans biofilms and to assess the effects on biofilm components. Methods Forty-eight-hour C. albicans (ATCC 90028) biofilms were treated under four conditions: (I) aPDT [Photodithazine (PDZ) (25 mg/L) for 20 min + Light-Emitting Diode (LED) (660 nm, 18 J/cm(2))], (II) DNase+aPDT [5 min with 20 U/mL DNase (Dn) I before aPDT], (III) sonication+aPDT [So, 7 W, 170-190 J before aPDT], (IV) Dn+So+aPDT. Ten successive aPDT applications were performed. Colony-forming units per milliliter (CFU/mL) was assessed after each application. After applications 1, 5, and 10, total and insoluble dry weight, soluble/insoluble proteins, water-soluble polysaccharides (WSP), alkali-soluble polysaccharides (ASP), and extracellular DNA (eDNA) were quantified. Biofilm architecture was analyzed by scanning electron microscopy (SEM). Results All groups showed significant CFU/mL reduction after ten aPDT applications compared to untreated control: aPDT (5.7 log(1)(0)), Dn+aPDT (5.5 log(1)(0)), So+aPDT (5.3 log(1)(0)), Dn+So+aPDT (5.3 log(1)(0)) (p < 0.05). Insoluble proteins decreased after the first application (46%), and soluble proteins after the fifth and tenth (45%; p <= 0.032). WSP and eDNA were significantly reduced (p <= 0.047). No significant differences were observed for total/insoluble dry weight, proteins, and ASP. SEM revealed wall deformities and fewer clusters. Conclusion Successive aPDT applications reduced CFU/mL, WSP, and eDNA in C. albicans biofilms regardless of pretreatment strategy. (AU) | |
| Processo FAPESP: | 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 24/00741-5 - Utilização da Zerumbona para potencializar a inativação fotodinâmica do biofilme de CANDIDA ALBICANS: avaliação em isolados clínicos resistentes ao fluconazol e em modelo murino de candidíase oral |
| Beneficiário: | Ana Cláudia Pavarina |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |